应用氢化物发生-原子荧光光谱法同时测定家禽内脏中的硒和锗含量。采用微波消解法用硝酸和过氧化氢对样品进行消解,以硫脲作为预还原剂,以溶于5 g·L-1氢氧化钾溶液中的20 g·L-1硼氢化钾溶液作为还原剂。硒、锗的质量浓度均在500 μg·L-1范围内与其荧光强度呈线性关系,检出限(3s/k)分别为0.001 08 μg·L-1和0.017 1 μg·L-1。硒、锗的加标回收率分别在98.9%~102%,97.4%~102%之间。

HG-AFS was applied to the simultaneous determination of selenium and germanium in poultry offal. The sample was digested with HNO3 and H2O2 in a microwave digestor. Thiourea was added to the sample solution as pre-reductant and 20 g·L-1 KBH4 solution (dissolved in 5 g·L-1 KOH solution) was added as reductant. Linear relationships between values of fluorescence intensity and mass concentration of selenium and germanium were found in the same range within 500 μg·L-1, with detection limits (3s/k) of 0.001 08 μg·L-1 and 0.017 1 μg·L-1, respectively. Values of recovery found by standard addition method were in the range of 98.9%-102% for selenium and 97.4%-102% for germanium.