Simultaneous Determination of 19 Polycyclic Aromatic Hydrocarbons in Barbecue Meat by GC-MS with Purification by GPC
摘 要
提出了凝胶色谱净化-气相色谱-质谱法同时测定烧烤肉中19种多环芳烃含量的方法。烧烤肉样品经正己烷-二氯甲烷(1+1)溶液超声提取30 min后,上清液经凝胶色谱净化,所得净化液用HP-5MS色谱柱分离,全扫描模式和选择离子监测模式测定。19种多环芳烃的峰面积与质量浓度在5~200 μg·L-1范围内呈线性关系,检出限(3S/N)在0.1~0.5 μg·kg-1之间。在20,40,80 μg·kg-1 3个浓度水平进行加标回收试验,19种多环芳烃的回收率在58.0%~122%之间,相对标准偏差(n=6)小于21.5%。
Abstract
A method of GC-MS with purification by gel permeation chromatography (GPC) for the simultaneous determination of 19 polycyclic aromatic hydrocarbons (PAH′s) in barbecue meat was proposed. The sample was extracted ultrasonically with mixture of n-hexane-dichloromethane (1+1) for 30 min, and the extract was purified by gel permeation chromatography. HP-5MS column was used for GC separation, and full-scanning mode as well as selected ion monitoring mode was adopted in MS determination. Linear relationships between values of peak area and mass concentration of the 19 PAH′s were kept in the same range of 5-200 μg·L-1,with detection limits (3S/N) in the range of 0.1-0.5 μg·kg-1.Tests for recovery were made at 3 concentration levels of 20, 40 and 80 μg·kg-1 of the mixed standards, values of recovery found were in the range of 58.0%-122% with RSD′s (n=6) less than 21.5%.
中图分类号 O657.63
所属栏目 工作简报
基金项目 国家自然科学基金(201147005)
收稿日期 2012/12/29
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联系人作者马玲(py45@163.com)
备注马玲(1987-),女,新疆人,硕士研究生,主要研究方向为色谱分析。
引用该论文: MA Ling,ZHANG Xu-long,LIU Yin,WANG Kuan-qiang,PENG Yang. Simultaneous Determination of 19 Polycyclic Aromatic Hydrocarbons in Barbecue Meat by GC-MS with Purification by GPC[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2014, 50(1): 72~75
马玲,张旭龙,刘银,王宽强,彭秧. 凝胶色谱净化-气相色谱-质谱法同时测定烧烤肉制品中19种多环芳烃[J]. 理化检验-化学分册, 2014, 50(1): 72~75
被引情况:
【1】郑香平,丁立平,陈志涛,吴文凡,赵建晖,陈春添, "高效液相色谱法测定蔬菜中的苯并[α]芘",理化检验-化学分册 51, 1437-1439(2015)
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