提出了高效液相色谱法测定农药中三氟氯菊酸含量的方法。样品以甲醇超声提取,所得提取液以Agilent Eclipse XDB-C18色谱柱为分离柱,以甲醇与磷酸(0.5+99.5)溶液以体积比55比45组成的混合液为流动相,在检测波长230 nm处进行测定。三氟氯菊酸质量浓度在0.025~0.5 g·L-1范围内与其峰面积呈线性关系,方法检出限(3S/N)为0.112 mg·L-1。加标回收率在97.5%~98.1%之间,测定值的相对标准偏差(n=6)小于2%。

A method of HPLC for the determination of lambda cyhalthrin acid in pesticide was proposed. The sample was extracted ultrasonically with methanol. The extract obtained was separated by using Agilent Eclipse XDB-C18 chromatographic column as stationary phase, and mixture of methanol and (φ) 0.5% phosphoric acid solution (55+45) as mobile phase. UV-detection at 230 nm was adopted in the determination. Linear relationship between values of peak area and mass concentration of lambda cyhalthrin acid was kept in the range of 0.025-0.5 g·L-1, with detection limit (3S/N) of 0.112 mg·L-1. Values of recovery found by standard addition method were in the range of 97.5%-98.1%, and values of RSD′s (n=6) less than 2%.