分光光度法间接快速测定过氧化氢——基于纳米α-FeOOH/GO的类似过氧化物酶的催化作用
Indirect and Rapid Spectrophotometric Determination of Hydrogen Peroxide——Based on the Catalytic Action of Mimetic Peroxidase of Nanoparticles of α-FeOOH/GO
摘 要
试验表明用超声原位沉淀法制备的α-FeOOH/GO纳米颗粒具有类似于辣根过氧化物酶的活性,且能催化活化过氧化氢而使底物N,N-二乙基-p-苯二胺氧化生成在550 nm处有吸收峰的有色化合物。在反应条件下,过氧化氢的浓度在2.0×10-7~2.0×10-4mol·L-1范围内与吸光度呈线性关系,检出限(3S/N)为1.0×10-7mol·L-1。据此提出了用分光光度法间接、快速测定过氧化氢含量的方法。应用此方法测定了雨水和蜂蜜样品中过氧化氢的含量,并用标准加入法进行回收试验,测得回收率在92.3%~108%之间,测定值的相对标准偏差(n=5)均小于5%。
α-FeOOH/GO纳米颗粒
类似过氧化物酶
催化作用
Spectrophotometry
Nanoparticles of α-FeOOH/GO
Mimetic peroxidase
Catalytic action
Abstract
It was found that nanoparticles of α-FeOOH/GO, prepared by ultrasonic in situ precipitation, behaved to have the activity mimetic to HRP and to catalytically activate H2O2, by which the base compound, N,N-diethyl-p-phenylenediamine was oxidized to give a colored compound with absorption maximum at 550 nm. Under the optimized reaction conditions. Linear relationship between values of absorbance and concentration of H2O2 was obtained in the range of 2.0×10-7-2.0×10-4mol·L-1, with detection limit (3S/N) of 1.0×10-7mol·L-1. Based on these facts a rapid spectrophotometric method for determination of H2O2 was proposed. The proposed method was applied to determination of H2O2 in samples of rainwater and honey. Recovery was tested by standard addition method, giving results of recovery in the range of 92.3%-108%, with values of RSD′s (n=5) less than 5%.
中图分类号 O657.3
所属栏目 工作简报
基金项目 国家自然科学基金项目(21107143;21207033);中南民族大学中央高校基本科研业务费专项资金项目资助(CZQ11019)
收稿日期 2013/3/11
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备注黄佳(1987-),女,湖北恩施人,硕士研究生,主要从事环境分析化学的研究。
引用该论文: HUANG Jia,CHANG Qing,JIANG Guo-dong,QIU Yang,TANG He-qing. Indirect and Rapid Spectrophotometric Determination of Hydrogen Peroxide——Based on the Catalytic Action of Mimetic Peroxidase of Nanoparticles of α-FeOOH/GO[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2014, 50(4): 417～420
黄佳,常青,江国栋,邱阳,唐和清. 分光光度法间接快速测定过氧化氢——基于纳米α-FeOOH/GO的类似过氧化物酶的催化作用[J]. 理化检验－化学分册, 2014, 50(4): 417～420
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参考文献
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【3】MANZOORI J, AMJADI M, OROOJI M. Application of crude extract of kohlrabi (brassica oleracea gongylodes) as a rich source of peroxidase in the spectrofluorometric determination of hydrogen peroxide in honey samples［J］. Anal Sci, 2006,22(9):1201-1206.
【4】李永新,赵丹华,卓淑娟,等.鲁米诺-四磺基锰酞菁-过氧化氢化学发光体系在蛋白质测定中的应用［J］.分析化学, 2003,31(5):638-638.
【5】HE Shao-hui, SHI Wen-bing, ZHANG Xiao-dan, et al. β-cyclodextrins-based inclusion complexes of CoFe2O4 magnetic nanoparticles as catalyst for the luminol chemiluminescence system and their applications in hydrogen peroxide detection［J］. Talanta, 2010,82(1):377-383.
【6】马美萍,连国军,曹建明.辣根过氧化物酶/多壁碳纳米管修饰铂电极生物传感器用于过氧化氢的循环伏安测定［J］.理化检验-化学分册, 2011,47(3):278-280.
【7】JIANG Guo-dong, TANG He-qing, ZHU Li-hua, et al. Improving electrochemical properties of liquid phase deposited TiO2 thin films by doping sodium dodecylsulfonate and its application as bioelectrocatalytic sensor for hydrogen peroxide［J］. Sens Actuator B Chem, 2009,138(2):607-612.
【8】陈志兵,王鹏,李艳,等.功能性碲化镉纳米荧光探针荧光猝灭法测定过氧化氢［J］.理化检验-化学分册, 2009,45(4):414-418.
【9】CHANG Qing, ZHU Li-hua, JIANG Guo-dong, et al. Sensitive fluorescent probes for determination of hydrogen peroxide and glucose based on enzyme-immobilized magnetite/silica nanoparticles［J］. Anal Bioanal Chem, 2009,395(7):2377-2385.
【10】CHEN Qiu-ying, LI Dong-hui, ZHU Qing-zhi, et al. Application of iron tetrasulfonatophthalocyanine as a new mimetic peroxidase in the determination of hydrogen peroxide with p-hydroxyphenylpropionic acid as a substrate［J］. Anal Chim Acta, 1999,381(2/3):175-182.
【11】WEI Hui, WANG Er-kang. Fe3O4 magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection［J］. Anal Chem, 2008,80(6):2250-2254.
【12】PENG Cheng, JIANG Bo-wei, LIU Qing, et al. Graphene-templated formation of two-dimensional lepidocrocite nanostructures for high-efficiency catalytic degradation of phenols［J］. Energy Environ Sci, 2011,4:2035-2040.
【13】HUMMERS W S, OFFEMAN R E. Preparation of graphitic oxide［J］. J Am Chem Soc, 1958,80(6):1339-1339.
【14】CHITRAKAR R, TEZUKA S, SONODA A, et al. Phosphate adsorption on synthetic goethite and akaganeite［J］. J Colloid Interface Sci, 2006,298(2):602-608.
【15】BADER H, STURZENEGGER V, HOIGN J. Photometric method for the determination of low concentrations of hydrogen peroxide by the peroxidase catalyzed oxidation of N,N-diethyl-p-phenylenediamine (DPD)［J］. Water Res, 1988,22(9):1109-1115.
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