基于一种分子内电荷转移(ICT)化合物,1-酮-2-(对二甲氨基苯亚甲基)-四氢萘(KDTN)与β环糊精(β-CD)所生成的包合物和牛血清白蛋白(BSA)形成三元超分子体系产生的荧光,且其荧光强度与BSA的质量浓度在3.31~662 mg·L-1范围内呈线性关系,检出限(3S/N)为1.6 mg·L-1,提出了以KDTN与β-CD包合物为新的荧光探针的荧光光谱法测定BSA含量。该反应体系的荧光发射峰位于540 nm,测定在pH 9.18的B-R缓冲介质中进行。用标准加入法进行回收试验,测得平均回收率为99.4%,测定值的相对标准偏差(n=11)为2.2%。

Based on the fact that a complex is formed between the intramolecular charge transferring (ICT) compound, 1-keto-2-(p-dimethylaminobenzal)-tetrabydronaphthalene (KDTN), and β-cyclodextrin (β-CD) and that in a B-R buffer medium of pH 9.18, a ternary supermolecular system is formed between the complex of KDTN with β-CD and the bovine serum albumin (BSA) which emits fluorescence at the emission maximum of 540 nm, a fluorophotometric method for determination of BSA using the complex of KDTN-β-CD as a new fluorescence probe was proposed. Linear relationship was kept between values of fluorescence intensity and mass concentration of BSA in the range of 3.3-662 mg·L-1, with detection limit (3S/N) of 1.6 mg·L-1. Recovery was tested by standard addition method, giving values of average recovery and RSD (n=11) of 99.4% and 2.2% respectively.