提出了亲水作用-高效液相色谱法测定不同烟草部位中烟碱含量的方法。采用Atlantis HILIC色谱柱(150 mm×4.6 mm,5 μm)分离,以乙腈、10 mmol·L-1乙酸铵溶液和冰乙酸以体积比72比28比2组成的混合液为流动相,在检测波长260 nm处进行测定。烟碱的质量浓度在0.001~0.1 g·L-1范围内与峰面积呈线性关系,方法检出限(3S/N)为0.17 mg·L-1。进行3个浓度水平的加标回收试验,测得回收率在98.2%~98.9%之间,相对标准偏差(n=6)小于2.0%。从烟草的不同部位取样,按本法分析,结果表明:其烟碱含量从烟叶的上部、中部、下部至烟梗逐渐减少。

A method of HPLC for the determination of nicotine in various parts of tobacco with hydrophilic chromatographic separation was proposed. The sample was separated on Atlantis HILIC chromatographic column (150 mm×4.6 mm, 5 μm), and a mixture of acetonitile, 10 mmol·L-1 ammonium acetate solution and glacial acetic acid (72+28+2) was used as mobile phase. UV-detection at 260 nm was adopted in the determination. Linear relationship between values of peak area and mass concentration of nicotine was kept in the range of 0.001-0.1 g·L-1, with detection limit (3S/N) of 0.17 mg·L-1. Tests for recovery were made by addition of standard solutions at 3 concentration levels, giving values of recovery in the range of 98.2%-98.9% with RSD′s (n=6) less than 2.0%. Samples were taken from upper, middle and lower part of the tobacco leaves as well as from the stems, and analyzed by the proposed method, the contents of nicotine were found to decrease in the respective order.