以亲水性离子液体溴化N-丁基吡啶(［BPy］Br)和磷酸氢二钾形成的双水相体系结合微波辅助萃取姜黄中姜黄素类化合物,在波长424.5 nm处测量其吸光度,提出了紫外分光光度法测定姜黄素类化合物总量。通过正交试验对离子液体双水相微波辅助提取姜黄中姜黄素的条件进行了试验,得到最佳提取条件:料液比为0.015比1,微波功率为320 W,提取时间为120 s,磷酸氢二钾用量为7.0 g。在此条件下,姜黄素的提取率达4.99%,比传统热回流提取的提取率(0.042%)高100多倍。

Microwave assisted extraction was applied to the separation of curcumin from curcuma longa L, in a bi-aqueous phase system containing ionic liquid of N-butylpyridine bromide (abbr. as ［BPy］Br) and K2HPO4. Its absorbance was measured at the ware length of 424.5 nm a UV spectrophotometric method for determination of the content of total curcumin was proposed. The optimum conditions of extraction were tested by the orthogonal experiment and the results found were as follows: ① mass ratio of sample to liquid: 0.015 to 1; ② microwave power: 320 W; ③ time of extraction: 120 s; and ④ amount of K2HPO4 added: 7.0 g. Under the optimized conditions, extraction rate of curcumin attained to a value of 4.99% which was more than 100 times higher than the extraction rate (0.042%) obtained by the conventional heat-reflux-extraction method.