顶空-固相微萃取-气相色谱-质谱法测定乳胶避孕套中亚硝胺的模拟迁移量
Determination of Amount of Simulated Migration of NNitrosamines from Condoms by GCMS with HSSPME
摘 要
采用顶空固相微萃取气相色谱质谱法测定了乳胶避孕套中8种亚硝胺的模拟迁移量。参照人工汗液配制方法,配制了酸度为pH 4.5的模拟阴道分泌液。称取避孕套碎片2 g于顶空瓶中,加入上述分泌液10 mL,在(40±2) ℃下磁力搅拌(10±0.5) min。选用CAR/PDMS纤维头在45 ℃下固相萃取60 min,随即将萃取头移至进样口解吸并进行气相色谱质谱分析。8种亚硝胺的检出限(3S/N)在0.03~0.70 μg·kg-1之间。应用此方法分析了8件样品,均检出N亚硝基二甲胺、N亚硝基二乙胺及N亚硝基二丁胺,总亚硝胺的迁移量为9.89~823.41 μg·kg-1。
顶空固相微萃取
N亚硝胺
模拟迁移
避孕套
GCMS
HSSPME
NNitrosamines
Simulative migration
Condoms
Abstract
GCMS with HSSPME was applied to the determination of 8 nitrosamines migrated from samples of condoms under simulated migration medium. Simulated vagina secretory juice (SVSJ) of pH 4.5 was prepared. Sample of condoms cut into pieces (2 g) was placed into a HS flask containing 10 mL of the SVSJ and stirred for (10±0.5) min at (40±2) ℃. CAR/PDMS microextractor was used and SPME was carried out at 45 ℃ for 60 min. The microextractor was then removed out and introduced into the sample inlet of the chromatograph for desorption of the analytes and for GCMS analysis. Values of detection limit (3S/N) of the 8 nitrosamines were found in the range of 0.03-0.70 μg·kg-1. Eight samples of condoms were analysed by the proposed method. Nnitrosodimethylamine (NDMA), Nnitrosodiethylamine (NDEA) and Nnitrosodibutylamine (NDBA) were detected. Total amounts of migration of nitrosamines were ranged from 9.89 to 823.41 μg·kg-1.
中图分类号 O657.63
所属栏目 <参考文献原文>
基金项目 国家自然科学基金资助项目(31301465);北京市优秀人才培养资助项目(2012D005003000001);科研基地建设科技创新平台国家自科基金配套(北京市级)(19005418040)
收稿日期 2013/6/20
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备注封棣(1980-),女,河北保定人,讲师,博士,主要从事色谱分离技术工作。
引用该论文: FENG Di,YI Xinru,YANG Huimin,ZHAO Liyuan. Determination of Amount of Simulated Migration of NNitrosamines from Condoms by GCMS with HSSPME[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2014, 50(6): 670～674
封棣,易馨如,杨慧敏,赵丽源. 顶空-固相微萃取-气相色谱-质谱法测定乳胶避孕套中亚硝胺的模拟迁移量[J]. 理化检验－化学分册, 2014, 50(6): 670～674
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参考文献
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【3】杨荣静,戴雪伟,卫碧文,等.固相萃取气相色谱质谱联用法检测婴儿奶嘴中11种N亚硝胺物质的迁移量［J］.环境化学, 2012,31(8):1283-1286．
【4】FENG Di, WANG Huiping, CHENG Xuelian, et al. Detection and toxicity assessment of nitrosamines migration from latex gloves in the Chinese market［J］. International Journal of Hygiene and Environmental Health, 2009,212:533-540．
【5】FENG Di, ZHOU Qingfeng, CHENG Xuelian, et al. Analysis of nitrosamines migration from condoms in the Chinese market using a proper migration experiment［J］. Bulletin of Environmental Contamination and Toxicology, 2010,84:373-377．
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【7】章若红,徐德佳,江艳,等.与食品接触橡胶制品中N亚硝胺的来源、危害及控制［J］.世界橡胶工业, 2011,38(4):33-36．
【8】BIAUDET H, MOUILLET L, DEBRY G. Migration of nitrosamines from condoms to physiological secretions［J］. Bulletin of Environmental Contamination and Toxicology, 1997,59:847-853．
【9】封棣,程雪莲,张苓利,等.中国市售天然乳胶避孕套中亚硝胺迁移量的检测分析［J］.中国卫生检验杂志, 2009,3:483-484．
【10】VENTANAS S, MARTIN D, ESTEVEZ M, et al. Analysis of volatile nitrosamines from a model system using SPMEDED at different temperatures and times of extraction［J］. Food Chemistry, 2006,99:842-850．
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【13】ANDRADE R, REYES F G R, RATH S. A method for the determination of volatile Nnitrosamines in food by HSSPMEGCTEA［J］. Food Chemistry, 2005,91:173-179．
【14】幸苑娜,王欣,陈泽勇,等.气相色谱正化学源质谱法测定家用橡胶手套中7种N亚硝胺及其前体物的迁移量［J］.分析测试学报, 2011,30(5):503-508．
【2】WERNE A, SRTEFAN B, KATHI E, et al. Migration of nitrosamines from rubber productsare balloons and condoms harmful to the human health［J］. Molecular Nutrition and Food Research, 2005,49:235-238．
【3】杨荣静,戴雪伟,卫碧文,等.固相萃取气相色谱质谱联用法检测婴儿奶嘴中11种N亚硝胺物质的迁移量［J］.环境化学, 2012,31(8):1283-1286．
【4】FENG Di, WANG Huiping, CHENG Xuelian, et al. Detection and toxicity assessment of nitrosamines migration from latex gloves in the Chinese market［J］. International Journal of Hygiene and Environmental Health, 2009,212:533-540．
【5】FENG Di, ZHOU Qingfeng, CHENG Xuelian, et al. Analysis of nitrosamines migration from condoms in the Chinese market using a proper migration experiment［J］. Bulletin of Environmental Contamination and Toxicology, 2010,84:373-377．
【6】幸苑娜,王欣,陈泽勇,等.气球中N亚硝胺及其前体物迁移量的测定与NDMA的儿童致癌暴露风险分析［J］.分析测试学报, 2012,31(7):779-784．
【7】章若红,徐德佳,江艳,等.与食品接触橡胶制品中N亚硝胺的来源、危害及控制［J］.世界橡胶工业, 2011,38(4):33-36．
【8】BIAUDET H, MOUILLET L, DEBRY G. Migration of nitrosamines from condoms to physiological secretions［J］. Bulletin of Environmental Contamination and Toxicology, 1997,59:847-853．
【9】封棣,程雪莲,张苓利,等.中国市售天然乳胶避孕套中亚硝胺迁移量的检测分析［J］.中国卫生检验杂志, 2009,3:483-484．
【10】VENTANAS S, MARTIN D, ESTEVEZ M, et al. Analysis of volatile nitrosamines from a model system using SPMEDED at different temperatures and times of extraction［J］. Food Chemistry, 2006,99:842-850．
【11】VENTANAS S, RUIZ J. Onsite analysis of volatile nitrosamines in food model systems by solidphase microextraction coupled to a direct extraction device［J］. Talanta, 2006,70:1017-1023．
【12】GREBEL J E, YOUNG C C, SUFFET I H. Solidphase microextraction of Nnitrosamines［J］. Journal of Chromatography A, 2006,1117:11-18．
【13】ANDRADE R, REYES F G R, RATH S. A method for the determination of volatile Nnitrosamines in food by HSSPMEGCTEA［J］. Food Chemistry, 2005,91:173-179．
【14】幸苑娜,王欣,陈泽勇,等.气相色谱正化学源质谱法测定家用橡胶手套中7种N亚硝胺及其前体物的迁移量［J］.分析测试学报, 2011,30(5):503-508．
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