聚2,6-二氨基-4,5,6,7-四氢苯并噻唑修饰玻碳电极伏安法测定多巴胺和尿酸
Voltammetric Determination of Dopamine and Uric Acid with Poly 2,6-diamino-4,5,6,7-tetrahydrobenzothiazole Modified Glassy Carbon Electrode
摘 要
将经过抛光、清洗的GCE置于1 mmol·L-1 2,6-二氨基-4,5,6,7-四氢苯并噻唑(DATHBT)溶液中,用循环伏安法以0.05 V·s-1扫描速率在-0.6~2.0 V电位范围内循环扫描15圈,制得PDATHBT薄膜修饰的玻碳电极(PDATHBT/GCE)。试验表明:该修饰电极对多巴胺(DA)和尿酸(UA)的电极反应具有催化作用,而且使原来在裸GCE上产生的DA和UA重叠的氧化峰分开成为两个独立的灵敏氧化峰,其氧化峰电流与DA和UA的浓度分别在0.1~100 μmol·L-1和2~1 000 μmol·L-1之间呈线性关系,两者的检出限(3S/N)依次为0.05,0.2 μmol·L-1。加标回收率在97.0%~100%之间(DA)和96.5%~110%之间(UA),测定值的相对标准偏差(n=6)均小于3.5%。
修饰电极
多巴胺
尿酸
Poly 2,6-diamino-4,5,6,7-tetrahydrobenzothiazole
Modified electrode
Dopamine
Uric acid
Abstract
Polished and cleaned GCE was placed into 1 mmol·L-1 2,6-diamino-4,5,6,7-tetrahydrobenzothiazole (DATHBT) solution, and treated by CV for 15 cycles at a rate of 0.05 V·s-1 in the potential range of -0.6 V to 2.0 V to prepare poly DATHBT membrane modified GCE (PDATHBT/GCE). It was found that catalytic oxidation of dopamine (DA) and uric acid (UA) was observed at this modified electrode, and 2 clearly separated and sensitive oxidation peaks of DA and UA were found, while in contrast to the bare GCE, over-lapped peaks of DA and UA were obtained. Linear relationships between values of the oxidation peak current and concentration of DA and UA were found in the ranges of 0.1-100 μmol·L-1 and 2-1 000 μmol·L-1 respectively with detection limits (3S/N) of 0.05 μmol·L-1 for DA and 0.2 μmol·L-1 for UA. Values of recovery found by standard addition method were in the ranges of 97.0%-100% for DA and 96.5%-110% for UA with RSD′s (n=6) all less than 3.5%.
中图分类号 O657.14
所属栏目 试验与研究
基金项目 上海市教育科学研究项目(B-13034)
收稿日期 2013/12/16
修改稿日期
网络出版日期
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备注张莹(1988-),女,吉林省吉林市人,硕士研究生,主要研究方向为电分析化学。
引用该论文: ZHANG Ying,ZHANG Lei. Voltammetric Determination of Dopamine and Uric Acid with Poly 2,6-diamino-4,5,6,7-tetrahydrobenzothiazole Modified Glassy Carbon Electrode[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2014, 50(7): 797～804
张莹,张雷. 聚2,6-二氨基-4,5,6,7-四氢苯并噻唑修饰玻碳电极伏安法测定多巴胺和尿酸[J]. 理化检验－化学分册, 2014, 50(7): 797～804
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参考文献
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【3】ZEN J M, JOU J J, ILANGOVAN G. Selective voltammetric method for uric acid detection using pre-anodized Nafion-coated glassy carbon electrodes［J］. Analyst, 1998,123:1345-1350.
【4】POPA E, KUBOTA Y, TRYK, D A, FUJISHIMA A. Selective voltammetric and amperometric detection of uric acid with oxidized diamond film electrodes［J］. Anal Chem, 2000,72(7):1724- 1727.
【5】ZHANG Lei, ZHANG Chun-hua, LIAN Ji-ying. Electrochemical synthesis of polyaniline nano-networks on p-aminobenzene sulfonic acid functionalized glassy carbon electrode: its use for the simultaneous determination of ascorbic acid and uric acid［J］. Biosens Bioelectron, 2008,24(4):690-695.
【6】ULUBAY 瘙塁, DURSUN Z. Cu nanoparticles incorporated polypyrrole modified GCE for sensitive simultaneous determination of dopamine and uric acid［J］. Talanta, 2010,80(3):1461-1466.
【7】黄文华,侯宝琴,张雷.聚钙羧酸修饰玻碳电极差分伏安法同时测定多巴胺和尿酸［J］.理化检验-化学分册, 2012,48(6):638-642.
【8】WENG Xue-xiang, CAO Qing-xue, LIANG Li-xin, et al. Simultaneous determination of dopamine and uric acid using layer-by-layer graphene and chitosan assembled multilayer films［J］. Talanta, 2013,117:359- 365.
【9】CAO Xiao-mei, LUO Li-qiang, DING Ya-ping, et al. Simultaneous determination of dopamine and uric acid on nafion/sodium dodecylbenzenesulfonate composite film modified glassy carbon electrode［J］. J Appl Electrochem, 2009,39(9):1603-1608.
【10】张银烽,华梅,赵晓慧,等.Pt-Au二元双金属/氧化石墨烯电化学传感器同时检测尿酸及多巴胺的应用研究［J］.化学传感器, 2012,32(3):43-50.
【11】霍瑞伟,贾丽萍,姚飞,等. Nafion-离子液体-碳纳米管复合膜修饰电极的制备及用于抗坏血酸、多巴胺、及尿酸的同时测定［J］. 中国科学, 2013,43(11):1572-1583.
【12】ZHANG Wen, YUAN Ruo, CHAI Ya-qin. A simple strategy based on lanthanum-multiwalled carbon nanotube nanocomposites for simultaneous determination of ascorbic acid,dopamine,uric acid and nitrite［J］. Sens Actuators B: Chemical, 2012,166:601-607.
【13】ENSAFI A A, TAEI M, KHAYAMIAN T. Simultaneous determination of ascorbic acid,dopamine,and uric acid by differential pulse voltammetry using tiron modified glassy carbon electrode［J］. Int J Electrochem Sci, 2010,5:116-130.
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【17】YANG Wei, ZHAO Jin-sheng, CUI Chuan-sheng, et al. Electrochemical synthesis and investigation of poly(1,4-bis(2-(3,4-ethylenedioxy)thienyl)benzene) and its application in an electrochromic device［J］. J Solid State Electrochem, 2012,16(12):3805-3815.
【18】SOLMAZ R, KARDA瘙塁 G. Electrochemical synthesis and characterization of poly-2-aminothiazole［J］. Prog Org Coat, 2009,64(1):81-88.
【19】GAO Zhi-qiang, HUANG Hai. Simultaneous determination of dopamine, uric acid and ascorbic acid at an ultrathin film modified gold electrode［J］. Chem Commun, 1998(19):2107-2108.
【20】LAVIRON E. General expression of the linear potential sweep voltammogram inthe case of diffusionless electrochemical systems［J］. J Electroanal Chem, 1979,101(1):19-28.
【21】FINKLEA H O, SNIDER D A, FEDYK J, et al. Characterization of octadecanethiol-coated gold electrodes as microarray electrodes by cyclic voltammetry and AC impedance spectroscopy［J］. Langmuir, 1993,9(12):3660-3667.
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