基于β-环糊精对荧光桃红的荧光增敏作用的开关型荧光探针测定痕量镍
Determination of Trace Amount of Nickel with a Fluorescence “Switching” Probe Based on Enhancement of Fluorescence of Phloxine by β-Cyclodextrin
摘 要
在pH7.6的PBS介质中,且在PVA存在下,荧光桃红在其荧光发射峰555 nm处的荧光强度因β-环糊精的加入而增强(荧光开),当在此体系中加入镍(Ⅱ)离子,其荧光发生猝灭(荧光关),其猝灭程度(ΔIf)与镍(Ⅱ)的质量浓度在5~300 μg·L-1范围内呈线性关系,检出限(3S/N)为1.75 μg·L-1。应用此“开关”型荧光探针测定了污水和矿石样品中镍的含量,并用标准加入法进行回收试验,测得回收率在98.8%~106%之间,测定值的相对标准偏差(n=10)在1.2%~2.2%之间。对此荧光体系的反应机理也作了初步探讨。
β-环糊精
“开关”型荧光探针
镍(Ⅱ)
Phloxine
β-Cyclodextrin
Fluorescence “switching” probe
Nickel(Ⅱ)
Abstract
In a PBS medium of pH 7.6 and in the presence of PVA, the fluorescence intensity (If) of phloxine at its fluorescence emission maximum of 555 nm was enhanced by addition of β-cyclodextrin (called “swtching in”), and was quenched by the presence of Ni(Ⅱ) ion (called “switching off”), and the magnitude of fluorescence quenching (ΔIf) was found to keep linear relationship with mass concentration of Ni(Ⅱ) in the range of 5-300 μg·L-1, with detection limit (3S/N) of 1.75 μg·L-1. This so-called fluorescence “switching” probe was used for fluorimetric determination of trace amount of Ni in waste water and ore samples, and recovery was tested by standard addition method, giving values of recovery and RSD′s (n=10) in the ranges of 98.8%-106% and 1.2%-2.2% respectively. A preliminary discussion on the mechanism of the fluorescence reaction system was also given.
中图分类号 O657.31
所属栏目 试验与研究
基金项目 广西自然科学基金项目(2011GXNS-FA018061);国家自然科学基金项目(21267008)
收稿日期 2013/6/26
修改稿日期
网络出版日期
作者单位点击查看
联系人作者陶慧林(thl@glut.edu.cn)
备注陶慧林(1961-),女,广西梧州人,副教授,研究方向为光谱分析。
引用该论文: TAO Hui-lin,XU Ming-ze,LIU Shuai-tao,LI Shu-huai,LIAO Xiu-fen,YI Zhong-sheng,ZHANG Qing-jun. Determination of Trace Amount of Nickel with a Fluorescence “Switching” Probe Based on Enhancement of Fluorescence of Phloxine by β-Cyclodextrin[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2014, 50(7): 815～818
陶慧林,徐铭泽,刘帅涛,黎舒怀,廖秀芬,易忠胜,张庆军. 基于β-环糊精对荧光桃红的荧光增敏作用的开关型荧光探针测定痕量镍[J]. 理化检验－化学分册, 2014, 50(7): 815～818
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【3】SHANG Yan, WU Fang-ying, QI Li. Highly selective colorimetric assay for nickel ion using N-acetyl-L-cysteine-functionalized silver nanoparticles［J］. Journal of Nanoparticle Research, 2012,14(10):1-7.
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【7】毛艳丽,肖晓存,潘建明,等.胞外高聚物分离富集-火焰原子吸收法测定水样中镍［J］.分析化学, 2011,39(7):1088-1092.
【8】屈颖娟,马明阳,李江涛,等.鲁米诺-H2O2-Cu(Ⅱ)化学发光法测定镍(Ⅱ)［J］.冶金分析, 2011,31(9):74-77.
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【10】李玉红,肖锋.催化荧光光度法测定痕量镍(Ⅱ)［J］.应用化工, 2012,41(10):1811-1813.
【11】AKSUNER N, HENDEN E, YILMAZ I, et al. A novel optical chemical sensor for the determination of nickel(Ⅱ) based on fluorescence quenching of newly synthesized thiazolo-triazol derivative and application to real samples［J］. Sensors and Actuators B: Chemical, 2012,166/167:269-274.
【12】崔新宇,谷红梅,牛莹莹,等.水杨基荧光酮-吐温-80-羟丙基-β-环糊精荧光猝灭法测定痕量锌［J］.冶金分析, 2011,31(9):67-69.
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【15】张敏,张宇昊,马良.β-环糊精及其衍生物、金属离子协同增敏黄曲霉毒素B1的荧光光谱分析及应用研究［J］.分析化学, 2011,39(12):1907-1911.
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