产氢颗粒污泥中聚-β-羟基丁酸酯(PHB)的紫外检测主要包括两步:污泥的预处理和紫外分光光度测定。样品经次氯酸钠法处理后,用二氯甲烷萃取,加入5 mL硫酸,水浴加热10 min,在波长235 nm处测量吸光度。残留的三氯甲烷蒸发60 min可除尽。PHB的质量浓度在4.0 mg·L-1以内与其吸光度呈线性关系,检出限(3s/k)为0.023 mg·L-1。方法用于产氢颗粒污泥的分析,加标回收率在90.5%~108%之间,测定值的相对标准偏差(n=6)小于2%。

Poly-β-hydroxybutyrate (PHB) in hydrogen-producing granular sludge was analyzed by ultraviolet (UV) spectrophotometry, which mainly included two steps: sludge pretreatment and UV determination. Sample of hydrogen-producing granular sludge was treated with NaClO and extracted with chloroform, 5 mL of H2SO4 solution was added and solution was heated in a water bath for 10 min, and its absorbance was measured at the wavelength of 235 nm. Linear relationship between values of absorbance and mass concentration of PHB was kept in the range within 4.0 mg·L-1 with detection limit (3s/k) of 0.023 mg·L-1. The proposed method was applied to the analysis of samples of hydrogen-producing granular sludge, giving values of recovery and RSD′s (n=6) in the ranges of 90.5%-108% and less than 2% respectively.