将制备好的氧化铝纳米通道经与3-氨丙基三乙氧基硅烷反应使其表面修饰了氨基后,再在含生物素的缓冲溶液(pH 5.5)中反应12 h,制成表面固定了生物素的氧化铝纳米通道,通道孔径约50 nm。另取PVC管一段,在其顶端用PVC/THF混合液粘附制备好的聚合物膜,再将上述修饰好的氧化铝纳米通道用有机硅橡胶粘在敏感膜的底部,作为工作电极待用。以生物素-亲和素体系为模型,用经修饰的氧化铝纳米通道为识别载体进行电位法检测,实现了亲和素的检测。亲和素的质量浓度在0.10~0.60 mg·L-1范围内与相应的电位变化值之间呈线性关系,检出限(3σ)为0.05 mg·L-1。试验结果验证了氧化铝纳米通道电位生物传感器测定生物大分子的可行性。

The perfectly ordered alumina nanochannels prepared by binary anodic oxidation was modified with amino-groups on the surface by reacting with 3-aminopropylethoxysilane and then reacted for 12 h in a buffer solution (pH 5.5) containing biotin to immobilize biotin on the surface of the nanochannels with bore diameters of ca. 50 nm. Circular slice of polymer membrane with  7 mm was glued with PVC/THF solution on the top of a length of PVC tubing, and the modified alumina nanochannels prepared was glued on the base of the membrane with organic silicone rubber, which was to be used as working electrode. Potentiometric study was made with biotin-avidin system as a mode, using the modified alumina nano-channels as discerning carrier. Linear relationship between values of potential change and mass concentration of avidin was kept in the range of 0.10 to 0.60 mg·L-1, with detection limit (3σ) of 0.05 mg·L-1. Feasibility of determination of bio-materials of macro-molecules using the modified alumina nanochannels as bio-sensor in potentiometry was proved.