采用高效液相色谱法测定橡胶制品中2-巯基苯并噻唑的含量。样品经丙酮超声提取两次。采用C18反相色谱柱为分离柱,以甲醇-0.01 mol·L-1磷酸二氢钠(80+20)溶液为流动相,在检测波长320 nm处进行测定。2-巯基苯并噻唑的质量浓度在0.10~10.0 mg·L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)为0.54 mg·kg-1。在0.10,2.0,10.0 mg·L-1等3个浓度水平进行加标回收试验,回收率在85.3%~95.4%之间,测定值的相对标准偏差(n=6)在3.1%~5.6%之间。

HPLC was applied to the determination of 2-mercatobenzothiozole(MBT) in rubber products. The sample was extracted ultrasonically with acetone twice. C18 reverse-phase chromatographic column was used as stationary phase, and a mixture of methanol-0.01 mol·L-1 NaH2PO4 solution (80+20) was used as mobile phase. The UV detector at 320 nm was used. Linear relationship between peak area and mass concentration of 2-MBT was in the range of 0.1-10.0 mg·L-1 with detection limit (3S/N) of 0.54 mg·kg-1. Recovery obtained by standard addition method at concentrations of 0.10, 2.0, 10.0 mg·L-1, and RSD′s (n=6) were in the ranges of 85.3%-95.4% and 3.1%-5.6%, respectively.