采用鸡肝酯酶作为生物敏感元件,以基于微机电加工技术制作的NCM9924微量热芯片作为换能器,建立了用于农药的残留量测定的量热式生物传感器试验系统。应用此系统进行了量热芯片灵敏度、酶促反应放热和农药抑制试验研究。结果表明:在流量为0.5 mL·min-1的情况下,换能器的温度灵敏度不低于1.184 mV·K-1;本系统对同一酶活水平的放热量响应具有良好的重现性。鸡肝酯酶被2 mg·L-1甲胺磷农药抑制后,系统放热曲线的峰高降低了50%。

Based on a MEMS microcalorimetric chip of model number NCM9924, a calorimetric biosensor experimental system for the determination of residual amounts of pesticide was established using chicken liver esterase as biological sensitive element. By means of this system, thermo-sensitivity of the calorimetric chip,heat release of the enzymatic reaction and inhibition by pesticide were studied. The results showed that temperature sensitivity of the transducer was not lower than 1.184 mV·K-1 at flow rate of 0.5 mL·min-1 and the reproducibility of the system response to heat release was good. The peak height of the heat release curve was reduced by 50% when the chicken liver esterase was inhibited by 2 mg·L-1 methamidophos.