铜掺杂ZnSe量子点荧光探针测定镉(Ⅱ)
Determination of Cadmium(Ⅱ) with Cu-Doped ZnSe Quantum Dots as Fluorescence Probe
摘 要
基于镉(Ⅱ)与ZnSe/Cu量子点之间的荧光猝灭作用,提出了用ZnSe/Cu量子点作为荧光探针测定镉的方法。通过微波辅助加热,在水溶液中合成了巯基丙酸修饰的ZnSe/Cu量子点。用荧光光谱、紫外光谱研究了ZnSe/Cu量子点与镉(Ⅱ)的相互作用。在pH为7.4的三羟甲基氨基甲烷-盐酸缓冲溶液和1.5×10-4mol·L-1 ZnSe/Cu量子点溶液中测定镉(Ⅱ)。镉(Ⅱ)的质量浓度在0.025~0.40 mg·L-1范围与ZnSe/Cu量子点荧光猝灭强度呈线性关系,方法的检出限(3s/k)为0.021 6 mg·L-1。方法应用于水样的测定,回收率为98.0%,102%。对0.15 mg·L-1镉(Ⅱ)标准溶液测定11次,测定值的相对标准偏差为6.7%。
荧光探针
镉(Ⅱ)
Quantum dots
Fluorescent probe
Cd(Ⅱ)
Abstract
A method for determination of cadmium(Ⅱ) with Cu-doped ZnSe quantum dots (ZnSe/Cu QDs) as fluorescence probe was proposed based on the fluorescence quenching effect of ZnSe/Cu QDs with cadmium(Ⅱ). ZnSe/Cu QDs modified with mercaptopropionic acid was synthesized with microwave-assisted heating in water solution. Interaction between ZnSe/Cu QDs and cadmium(Ⅱ) was investigated by fluorescence spectra and UV spectra. Cadmium(Ⅱ) was determined in Tris-HCl buffer solution of pH 7.4 and 1.5×10-4mol·L-1 ZnSe/Cu QDs solution. Linear relationship between values of fluorescence quenching intensity of ZnSe/Cu QDs and mass concentration of cadmium(Ⅱ) was obtained in the range of 0.025-0.40 mg·L-1, with detection limit (3s/k) of 0.021 6 mg·L-1. The proposed method was used to determination of water samples with values of recovery of 98.0% and 102%. Precision of the method was tested with 0.15 mg·L-1 cadmium(Ⅱ) standard solution for 11 determination, value of RSD found was 6.7%.
中图分类号 O657.32
所属栏目 工作简报
基金项目 广西自然科学基金项目(2011GXNSFA018045);广西研究生教育创新计划项目(2009105960703M17)
收稿日期 2013/11/26
修改稿日期
网络出版日期
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备注侯明(1957-),女,山东邹城人,教授,博士,研究方向为环境分析化学。
引用该论文: HOU Ming,YAN Xiao-ya. Determination of Cadmium(Ⅱ) with Cu-Doped ZnSe Quantum Dots as Fluorescence Probe[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2014, 50(12): 1508～1512
侯明,颜小娅. 铜掺杂ZnSe量子点荧光探针测定镉(Ⅱ)[J]. 理化检验－化学分册, 2014, 50(12): 1508～1512
被引情况:
【1】张雪梅,陈 飞,崔书亚,何志坚,杜军良, "功能化温控离子液体萃取-火焰原子吸收光谱法测定痕量镉",理化检验－化学分册 51, 1501-1504(2015)
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【2】张明英,宋娟.5-Br-DMPAP-OP双波长吸光光度法测定微量锌镉［J］.理化检验-化学分册, 1994,30(5):292-293.
【3】HASAN C, MEHMET E K. Determination of cadmium levels in agricultural areas of carsamba and Bafra plains［J］. Environ Monit Assess, 2007,132(1/3):165-169.
【4】陈秋生,刘烨潼,张强,等.电感耦合等离子体质谱法同时测定葡萄中40种痕量元素［J］.理化检验-化学分册, 2013,49(6):686-690.
【5】陈芙,方军,吴升海.基于阳极溶出伏安法(HM-3000P)测定食品中镉的应用［J］.现代科学仪器, 2013(1):16-18.
【6】JOHNSON D K. A fluorescence polarization immunoassay for cadmium(Ⅱ)［J］. Anal Chim Acta, 1999,399(1/2):161-172.
【7】DARWISH I A, BLAKE D A. Development and validation of a one-step immunoassay for determination of cadmium in human serum［J］. Analytical Chemistry, 2002,74(1):52-58.
【8】KHOSRAVIANI M, PAVLOV A R, FLOWERS G C, et al. Detection of heavy metals by immuno-assay: optimization and validation of a rapid, portable assay for ionic cadmium［J］. Environ Sci Technol, 1998,32(1):137-142.
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【10】LI Hai-bing, ZHANG Yan, WANG Xiao-qiong. l-Carnitine capped quantum dots as luminescent probes for cadmium ions［J］. Sensors and Actuators B: Chemical, 2007,127(2):593-597.
【11】HUANG Feng-hua, CHEN Guo-nan. Preparation and application ofl-cysteine-modified CdSe/CdS core/shell nanocrystals as a novel fluorescence probe for detection of nucleic acid［J］. Spectrochimica Acta Part A, 2008,70(2):318-323.
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【13】杜保安,刘澄,曹雨虹,等.CdTe量子点作荧光探针检测微量铅的方法研究［J］.光谱学与光谱分析, 2013,33(5):1266-1269.
【14】GAO Xue, WANG Chao, NIU Lu, et al. Aqueous synthesis of Cu-doped ZnSe quantum dots［J］. Journal of Luminescence, 2011,131(7):1300-1304.
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【17】WANG Chao, GAO Xue, MA Qiang, et al. Aqueous synthesis of mercaptopropionic acid capped Mn2+-doped ZnSe quantum dots［J］. Journal of Materials Chemistry, 2009,19:7016-7022.
【18】TU Ren-yong, LIU Bian-hua, WANG Zhen-yang, et al. Amine-capped ZnS-Mn2+ nanocrystals for fluorescence detection of trace TNT explosive［J］. Analytical Chemistry, 2008,80(9):3458-3465.
【19】HE Yu, WANG He-fang, YAN Xiu-ping. Exploring Mn-doped ZnS quantum dots for the room-temperature phosphorescence detection of enoxacin in biological fluids［J］. Analytical Chemistry, 2008,80(10):3832-3837.
【20】LIU Jun-xiao, CHEN Hui, LIN Zhen, et al. Preparation of surface imprinting polymer capped Mn-doped ZnS quantum dots and their application for chemiluminescence detection of 4-nitrophenol in tap water［J］. Analytical Chemistry, 2010,82(17):7380-7386.
【21】陈启凡,杨冬芝,徐淑坤,等.微波辐射法制备水溶性CdTe量子点及其光谱学研究［J］.光谱学与光谱分析, 2007,27(4):650-653.
【22】沈坤,侯明,那佳.微波辐射法制备水溶性CdTe/ZnS量子点及其与司帕沙星的作用［J］.分析试验室, 2011,30(1):5-9.
【23】YANG Ping, LU Meng-kai, ZHOU Guang-jun, et al. Photoluminescence characteristics of ZnS nanocrystallites co-doped with Co2+ and Cu2+［J］. Inorganic Chemistry Communications, 2001,4(12):734-737.
【24】杨淑平,金鑫,郑佳,等.高性能CdTe/CdS核壳型量子点的制备及应用于小麦面粉中呕吐毒素的荧光免疫检测研究［J］.化学学报, 2011,69(6):687-692.
【25】ALI E M, ZHENG Yuan-gang, YU H H, et al. Ultrasensitive Pb2+ detection by glutathione-capped quantum dots［J］. Analytical Chemistry, 2007,79(24):9452-9458.
【26】戚丽,吴芳英.ZnS:Mn量子点作为荧光离子探针测定痕量铜(Ⅱ)［J］.南昌大学学报:理科版, 2012,36(1):50-53.
【27】杨曼曼,杨频,张立伟.荧光法研究咖啡酸类药物与白蛋白的作用［J］.科学通报, 1994,39(1):31-35.
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