Rapid Determination of Salicylic Acid in Cosmetics by UPLC-MS/MS
摘 要
提出了超高效液相色谱-串联质谱法测定化妆品中水杨酸含量的方法。化妆品试样用甲醇溶解,振荡提取20 min,离心分离取上清液过ACQUITYTM BET C18色谱柱(2.1 mm×100 mm,1.7 μm)并用乙腈和甲酸-水(0.3+99.7)溶液(体积比6比4)的混合溶液作为流动相进行分离,串联质谱法进行测定。采用正离子模式多反应监测,同位素内标法定量。水杨酸的线性范围为1.0~5.0×103μg·L-1,方法的检出限(3S/N)为0.15 μg·L-1,测定下限(10S/N)为0.50 μg·L-1。方法用于分析8种化妆品试样,回收率在97.3%~107.0%之间,相对标准偏差(n=5)在2.0%~3.8%之间。
Abstract
A rapid method of UPLC-MS/MS for the determination of salicylic acid in cosmetics was proposed. The sample was dissolved with methanol and extracted vortically for 20 min. After centrifugation, supernatant was taken for separation by using the column ACQUITYTM BET C18 (2.1 mm×100 mm, 1.7 μm) and using the mixed solution of acetonifrile and formic acid water solution (0.3+99.7), mixed in the ratio of 6 to 4, as mobile phase. The content of salicylic acid was determined by MS/MS. The positive electrospray ionization as well as multiple reactions monitoring mode was used in the detection. The isotope internal standard method was used for quantification of salicylic acid. Linearity range of salicylic acid was kept from 1.0 to 5.0×103μg·L-1, with limit of detection (3S/N) of 0.15 μg·L-1 and limit of determination (10S/N) of 0.50 μg·L-1. The proposed method was applied to the analysis of 8 cosmetic samples, giving values of recovery in the range from 97.3%-107.0% and RSD (n=5) in the range from 2.0%-3.8%.
中图分类号 O657.63
所属栏目
基金项目
收稿日期 2010/2/4
修改稿日期
网络出版日期
作者单位点击查看
备注张瑞(1984-),女,河北保定人,硕士研究生,主要从事烟草化学研究。
引用该论文: ZHANG Rui,LU She-ming,DING Li-ting,ZHAO Yun-fang,WU Xing-hua,MENG Zhao-yu. Rapid Determination of Salicylic Acid in Cosmetics by UPLC-MS/MS[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2011, 47(1): 65~67
张瑞,陆舍铭,丁丽婷,招云芳,吴新华,孟昭宇. 超高效液相色谱-串联质谱法快速测定化妆品中水杨酸[J]. 理化检验-化学分册, 2011, 47(1): 65~67
被引情况:
【1】杨春林,李佳峻,李洪浩,吕小艇,胡强,吴蔚,刘昊, "分散萃取-高效液相色谱法同时测定化妆品中6种限量添加剂",理化检验-化学分册 49, 542-545(2013)
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】刘巧茄,石起增,董文举,等.高效离子色谱法测定水杨酸[J].分析科学学报, 2006,22(3):357-358.
【2】胡家元,乔秀明,李贤均.气相色谱法测定医用苯甲酸软膏中的苯甲酸与水杨酸含量[J].化学研究与应用, 2000,12(5):557-559.
【3】郑维国,果家言.双波长系数倍率法测定复方苯甲酸酊中苯甲酸和水杨酸的含量[J].实用医药杂志, 2007,24(7):827-828.
【4】柳松,谢伟平.化妆品中苯甲酸、山梨酸和水杨酸的测定[J].检验检疫科学, 2002,12(5):52-53.
【5】董煜,赵远征,张怡娜.高效液相色谱法测定注射用赖氨匹林中的阿司匹林及游离水杨酸[J].色谱, 2002,20(3):277-278.
【6】彭翱,周辉,江骥,等.LC-MS/MS法定量测定人血浆中阿司匹林及其代谢产物水杨酸[J].分析测试学报, 2007,26:4-6.
【7】LI Xiao-qin, XIONG Zhi-li, YING Xi-xiang, et al. A rapid ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric method for the qualitative and quantitative analysis of the constituents of the flower of trollius ledibouri reichb[J]. Anal Chim Acta, 2006,580:170-180.
【8】HUERTA-FONTELA M, GALCERAN M T, VENTURA F. Ultraperformance liquid chromatography-tandem mass spectrometry analysis of simulatory drugs ofabuse in wastewater and surface waters[J]. Anal Chem, 2007,79:3821-3829.
【2】胡家元,乔秀明,李贤均.气相色谱法测定医用苯甲酸软膏中的苯甲酸与水杨酸含量[J].化学研究与应用, 2000,12(5):557-559.
【3】郑维国,果家言.双波长系数倍率法测定复方苯甲酸酊中苯甲酸和水杨酸的含量[J].实用医药杂志, 2007,24(7):827-828.
【4】柳松,谢伟平.化妆品中苯甲酸、山梨酸和水杨酸的测定[J].检验检疫科学, 2002,12(5):52-53.
【5】董煜,赵远征,张怡娜.高效液相色谱法测定注射用赖氨匹林中的阿司匹林及游离水杨酸[J].色谱, 2002,20(3):277-278.
【6】彭翱,周辉,江骥,等.LC-MS/MS法定量测定人血浆中阿司匹林及其代谢产物水杨酸[J].分析测试学报, 2007,26:4-6.
【7】LI Xiao-qin, XIONG Zhi-li, YING Xi-xiang, et al. A rapid ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric method for the qualitative and quantitative analysis of the constituents of the flower of trollius ledibouri reichb[J]. Anal Chim Acta, 2006,580:170-180.
【8】HUERTA-FONTELA M, GALCERAN M T, VENTURA F. Ultraperformance liquid chromatography-tandem mass spectrometry analysis of simulatory drugs ofabuse in wastewater and surface waters[J]. Anal Chem, 2007,79:3821-3829.
相关信息