提出了高效液相色谱法同时测定复方草珊瑚含片中原儿茶酸、3-羟基-4-甲氧基苯甲酸、异嗪皮啶和迷迭香酸的含量。样品经甲醇超声提取,用Waters SunFire ODS色谱柱(4.6 mm×150 mm,5 μm)分离,以不同比例混合的乙腈和磷酸-水(0.1+99.9)溶液为流动相进行梯度洗脱,流量0.8 mL·min-1,在294 nm波长处用紫外检测器检测。在优化的色谱条件下,4种活性成分可完全分离。原儿茶酸、3-羟基-4-甲氧基苯甲酸、异嗪皮啶和迷迭香酸的线性范围分别为2.508~50.16,18.40~368.0,6.375~127.5,41.04~820.8 ng;平均回收率分别为92.8%,93.1%,91.9%,99.6%;相对标准偏差(n=6)均不大于5.0%。

HPLC was applied to the determination of 4 activitive components, namely, procatechuic acid, 3-hydroxy-4-methoxy benzoic acid, isofraxindin and rosmarinic acid, in sucking tablets of “Caoshanhu” compound. The sample was extracted ultrasonically with methanol and the extract was used for the determination. Water SunFire ODS column (4.6 mm×150 mm, 5 μm) was used as stationary phase, and mixtures of acetonitrile and phosphoric acid (0.1+99.9) solution in various ratios were used as mobile phase in gradient elution with flow-rate of 0.8 mL·min-1. Wavelength of 294 nm was chosen for UV-detection of the 4 components. Under optimum chromatographic conditions, linearity ranges were obtained between 2.508-50.16 ng (for procatechuic acid), 18.40-368.0 ng (for 3-hydroxy-4-methoxy benzoic acid), 6.375-127.5 ng (for isofraxindin) and 41.04-820.8 ng (for rosmarinic acid). Values of average recovery found were 92.8%,93.1%, 91.9% and 99.6% respectively. Values of RSD′s (n=6) found were less than or equal to 5.0%.