荧光分光光度法测定水中痕量钴和镍
Fluorospectrophotometric Determination of Trace Amounts of Co and Ni in Water
摘 要
试验合成了一种荧光试剂对-氨-苯基卟啉(TAPP),该试剂与钴(Ⅱ)离子和镍(Ⅱ)离子反应发生荧光淬灭,据此试验了以其为探针测定痕量钴(Ⅱ)和镍(Ⅱ)的荧光分光光度法。在pH 7.2的Tris-盐酸缓冲介质中,在一定量的十二烷基硫酸钠(SDS)存在下,通过测定样品溶液和空白溶液在激发波长392 nm和发射波长516 nm处的发射荧光强度Fs及F0,计算得反应液荧光强度减弱程度ΔF,其结果表明:ΔF与钴(Ⅱ)和镍(Ⅱ)的浓度均在2.0×10-7~1.0×10-5mol·L-1范围内呈线性关系,方法的检出限(3S/N)分别为2.0×10-8mol·L-1和4.0×10-8mol·L-1。此方法用于自然水体和污水中钴(Ⅱ)和镍(Ⅱ)的测定,回收率分别在97.0%~108.5%和97.0%~106.5%之间。
荧光探针
对-氨-苯基卟啉
镍(Ⅱ)离子
钴(Ⅱ)离子
Fluorospectrophotometry
Fluorescence probe
TAPP
Ni(Ⅱ)
Co(Ⅱ)
Abstract
A fluorescence reagent, 5,10,15,20-tetra(4-aminophenyl)-porphyrin (TAPP) was synthesized. Fluorescence quenching effect was observed when TAPP was reacted with Co(Ⅱ) or Ni(Ⅱ). The use of TAPP as a fluorescence probe in the determination of trace amounts of Co and Ni was studied. In a Tris-HCl buffer medium of pH 7.2, Co(Ⅱ) and Ni(Ⅱ) were reacted with TAPP in the presence of SDS, the magnitude of decrease in fluorescence intensity, ΔF, of the reaction was obtained by taking the difference between values of emission fluorescence intensity of the blank solution (F0) and the sample solution (Fs) measured at (λem) 516 nm, with (λex) at 392 nm. Linear relationships between values of ΔF and concentration of Co(Ⅱ) and Ni(Ⅱ) were found to be same in the range of 2.0×10-7-1.0×10-5mol·L-1, with their detection limits (3S/N)of 2.0×10-8mol·L-1 and 4.0×10-8mol·L-1 respectively. Values of recovery found in the analysis of samples of natural water and waste water, were in the range of 97.0 %-108.5 % for Co(Ⅱ) and 97.0%-106.5% for Ni(Ⅱ).
中图分类号 O657.32
所属栏目
基金项目 国家“863”项目(2006AA06Z407);湖南省自然科学基金(06JJ20062)
收稿日期 2010/2/17
修改稿日期
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备注彭博(1985-),男,湖南汨罗人,硕士,从事环境分析化学研究。
引用该论文: PENG Bo,NIU Cheng-gang,QIN Pin-zhu,RUAN Min,LI Hui-xia,ZHANG Yan. Fluorospectrophotometric Determination of Trace Amounts of Co and Ni in Water[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2011, 47(3): 302～304
彭博,牛承岗,秦品珠,阮敏,李慧霞,张妍. 荧光分光光度法测定水中痕量钴和镍[J]. 理化检验－化学分册, 2011, 47(3): 302～304
被引情况:
【1】陶慧林,黎舒怀,徐铭泽,周素莲,韦柳丹, "荧光猝灭法测定中草药中痕量钴",理化检验－化学分册 49, 413-416(2013)
【2】王妍,贾红霞,曹雨虹,马刚,杜保安, "基于硫化镉量子点的荧光猝灭效应测定微量钴离子",理化检验－化学分册 52, 1123-1126(2016)
【3】斯琴高娃,王立华,王宁,陈梦琪,陈葛, "铬黑T分光光度法测定饮料中痕量镍",理化检验－化学分册 49, 735-737(2013)
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参考文献
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【2】BAG H, TRKER A R, LALE M. Determination of Cu, Zn, Fe, Ni and Cd by flame atomic absorption spectrophotometry after preconcentration by Escherichia coli immobilized on sepiolite［J］. Talanta, 2000,51:1035-1043.
【3】黄青瑜,廖晓军.钴镍-水杨基荧光酮双波长光度法的研究［J］.冶金分析, 1991,11(4):55-56.
【4】FABBRIZZI L, LICCHELLI M, PALLAVICINI P. A Zinc(Ⅱ)-driven intramolecular photoinduced electron transfer［J］. Inorganic Chemistry, 1996,35:1733-1736.
【5】MANCEAU A, LANSON M, GEOFFROY N. Natural speciation of Ni, Zn, Ba, and As in ferromanganese coatings on quartz using X-ray fluorescence, absorption, and diffraction［J］. Geochimica et Cosmochimica Acta, 2007,71:95-128.
【6】KILBRIDE C, POOLE J, HUTCHINGS T R. A comparison of Cu, Pb, As, Cd, Zn, Fe, Ni and Mn determined by acid extraction/ICP-OES and ex situ field portable X-ray fluorescence analyses［J］. Environmental Pollution, 2006,143:16-23.
【7】GORDEEVA V P, STATKUS M A, TSYSIN G I, et al. X-ray fluorescence determination of As, Bi, Co, Cu, Fe, Ni, Pb, Se, V and Zn in natural water and soil extracts after preconcentration of their pyrrolidinedithiocarbamates on cellulose filters［J］. Talanta, 2003,61:315-329.
【8】BELIKOVA K N, BLANKA A B, SHEVTSOVA N I, et al. X-ray fluorescence determination of mobile forms of Cu, Zn and Co in soils with preconcentration on silica gel modified by didecylaminoethyl-β-tridecylammonium iodide［J］. Anal Chim Acta, 1999,383:277-281.
【9】THOMPSON R B, GE Zheng-fang, PATCHAN M, et al. Fiber optic biosensor for Co(Ⅱ) and Cu(Ⅱ) based on fluorescence energy transfer with an enzyme transducer［J］. Biosensors and Bioelectronics, 1996,11:557-564.
【10】朱宝库,徐志康,徐又一.四(4-硝基苯基)卟啉和四(4-氨基苯基)卟啉的合成［J］.应用化学, 1999,16(1):68-70.
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