提出了反相高效液相色谱法测定虾肌肉组织中阿维菌素残留量的方法。试样经乙酸乙酯提取,正己烷脱脂后,离心分离取下层溶液过Hypersil C18色谱柱(150 mm×4.6 mm,5 μm)分离,用甲醇-水(86+14)溶液为流动相,于245 nm波长处进行紫外检测。阿维菌素的质量浓度在0.005~2 mg·L-1范围内与峰面积呈线性关系,方法的检出限(3S/N)为0.002 mg·kg-1。以虾肌肉组织样品为基体,加入3种不同浓度水平的阿维菌素标准溶液作回收试验,测得回收率在89.4%~102.4%之间;日内相对标准偏差(n=6)和日间相对标准偏差(n=6)分别在2.1%~7.2%和5.5%~8.4之间。

It was reported that residual amount of abamectin in muscle tissues of shrimps was determined by RP-HPLC. Samples were extracted with ethyl acetate, and defatted with n-hexane. After centrifugation, an aliquot of the lower layer was taken for separation by passing through Hypersil C18 column (150 mm×4.6 mm, 5 μm). A mixture of methanol and water mixed in the ratio of 86 to 14 (by volume) was used as mobile phase; and UV-detection at 245nm was used in the determination. Linear relationship between values of peak area and mass concentration of the abamectin was kept in the range of 0.005-2 mg·L-1, with detection limit (3S/N) of 0.002 mg·kg-1. Test for recovery was made by addition of standard abamectin at 3 different concentration levels to samples of muscle tissues of shrimps as matrixes, values of recovery found were in the range of 89.4% to 102.4%. Values of intraday and interday relative standard deviation (n=6) found were in the ranges of 2.1%-7.2% and 5.5%-8.4% respectively.