提出了一种基于表面等离子体共振技术(SPR)测定肉类食品中泰乐菌素和磺胺类抗生素残留量的方法。用传感芯片(CM5),以乙腈-0.15 mol·L-1氢氧化钠(9+1)混合溶液为再生溶液,HBS-EP为缓冲溶液,流量为60 μL·min-1。结果表明:泰乐菌素、磺胺类抗生素的质量浓度分别在1~32,0.5~50 μg·L-1范围内,在传感芯片表面所产生的相对共振强度与目标分子浓度呈线性关系。检出限(3S/N)分别为0.27,4.5 μg·kg-1。泰乐菌素和磺胺类抗生素平均回收率分别为80.0%~98.8%和77.3%~98.4%,相对标准偏差(n=6)分别为4.8%和2.0%。

Method for determination of residual amounts of tylosin and sulfonamides in meat by application of the surface plasma resonance technology was proposed. The sensor chip of CM5 was used as the resonance chip; a mixture of acetonitrile and 0.15 mol·L-1 NaOH solution (9+1) was used as the regeneration solution; and HBS-EP was used as the buffer solution with its flow-rate of 60 μL·min-1. It was found that the values of relative intensity of resonance produced on the surface of the sensor chip CM5 kept linear relationship with mass concentration of tylosin and sulfonamides in the ranges of 1-32 μg·L-1 and 0.5-50 μg·L-1 respectively, with values of detection limits (3S/N) of 0.27 μg·kg-1 (for tylosin) and 4.5 μg·kg-1 (for sulfonamides). Recovery was tested, giving values of recovery in the ranges of 80.0%-98.8% (for tylosin) and 77.3%-98.4% (for sulfonamides). RSD′s (n=6) found were 4.8% and 2.0% respectively.