将1.0 g·L-1聚苯胺纳米管-N,N-二甲基甲酰胺(DMF)溶液和1.0 g·L-1壳聚糖-乙酸溶液以1比50的比例混合,并超声处理1 h后滴涂在玻碳电极表面,制得聚苯胺纳米管/壳聚糖修饰电极(记为PANT′s/CTS/GCE)。采用循环伏安法研究了阿米卡星在聚苯胺纳米管/壳聚糖修饰电极上的电化学行为。试验结果表明：在pH 5.0磷酸盐缓冲溶液中,阿米卡星在聚苯胺纳米管/壳聚糖修饰电极于-0.2 V处出现一个不可逆的还原峰,且还原峰电流与阿米卡星的质量浓度在10.0~80.0 mg·L-1范围内呈线性关系,检出限(3S/N)为8.0 mg·L-1。应用此法测定注射液中阿米卡星的含量,测定结果与分光光度法测定值相一致。

1.0 g·L-1 Polyaniline nanotubes-DMF solution was mixed with 1.0 g·L-1 chitosan-HOAc solution in the ratio of 1 to 50 and treated ultrasonically for 1 h. The mixed solution obtained was dropped on the surface of glassy carbon electrode (GCE), to prepare the modified electrode designated abbreviately as PANT′s/CTS/GCE. The electrochemical behavior of amikacin at this modified electrode was studied by cyclic voltammetry. It was found that an irreversible reduction peak was observed at -0.2 V in PBS buffer solution of pH 5.0. Linear relationship between values of reductive peak current and concentration of amikacin was obtained in the range of 10.0-80.0 mg·L-1, with detection limit (3S/N) of 8.0 mg·L-1. The proposed method was used in the determination of amikacin in injections, and the results obtained were in consistency with the results obtained by spectrophotometry.