应用高效液相色谱法测定了3种非法作为色素加入于食品中的碱性橙染料即碱性橙2(CDG)、碱性橙21(AOG)及碱性橙22(AOR)。将均质样品(20.00 g)与无水硫酸钠2 g混合后,用无水乙醇超声提取3次,每次用30 mL。将提取液合并,先在45 ℃真空蒸发,最后用吹氮法蒸干。用流动相(0.02 mol·L-1乙酸铵溶液与甲醇按39比61的比例混合)溶解残渣并定容至5 mL。将此溶液离心分离,取其上清液用于高效液相色谱分析。Agilent C18色谱柱用于各组分的分离。用二极管阵列检测器在430 nm波长处检测CDG,在480 nm波长处检则AOG及AOR,3种染料的线性范围均在0.50~20.0 mg·L-1之间,方法的检出限(3S/N)均为0.10 mg·L-1。应用此方法分析了黄鱼样品,并以此为基体加入3种染料的标准溶液后进行分析,测得其平均回收率依次91.2%,93.6%及96.0%,测定值的相对标准偏差(n=6)均小于2%。

HPLC was applied to the determination of 3 alkali orange dyestuffs, namely chrysoidine G (CDG), astrazon orange G (AOG) and astrazon orange R (AOR), which were added contrabandly to foodstuffs as colorants. The homogenized sample (20.00 g) was mixed with 2 g of anhydrous Na2SO4 and extracted thrice ultrasonically with absolute alcohol (30 mL+30 mL+30 mL). The extracts were combined and evaporated first at 45 ℃ in vacuum and then by N2-blowing to dryness. The residue was taken up with the mobile phase (mix 0.02 mol·L-1 NH4OAc solution with methanol in the ratio of 39+61 by volume) and made up to 5 mL. After centrifugation, the supernatant was used for HPLC analysis by separation on Agilent C18 column. Diode array detector was used for the determination of CDG at 430 nm and of AOG as well as AOR at 480 nm. Linearity ranges for the 3 dyestuffs were found to be same between 0.50 to 20.0 mg·L-1, with detection limits (3S/N) of 0.10 mg·L-1. Tests for recovery and precision were performed by addition of standards to a sample of yellow croaker, and the results of average recovery found were 91.2%, 93.6% and 96.0% for the 3 dyestuffs respectively, with RSD′s (n=6) less than 2%.