采用分子排阻色谱法,利用3台不同的液相色谱仪及相应GPC软件测定并计算β-葡聚糖受试物的相对分子质量及其分布。选择的色谱条件为SB-804HQ凝胶色谱柱(7.8 mm×300 mm),流动相为0.2 g·L-1叠氮化钠溶液,流量0.5 mL·min-1,柱温35 ℃,进样量25 μL,2410示差折光检测器(RI),以系列标准葡聚糖Shodex STANDARD P-82为相对分子质量标准品。灰树花GF-932发酵菌丝体β-葡聚糖的重均相对分子质量(Mr)在134 195~251 053 amu范围内,相对分子质量分布宽度(Mr/Mn)在1.64~2.61之间。受试物灰树花菌丝体β-葡聚糖是具有一定分子质量分布范围的高纯度的聚合物。

Values of relative molecular mass (Mr) and distribution of Mr of β-glucan were determined and calculated by using 3 chromatographs of different types with their respective GPC softwares. The chromatographic conditions used were as follows: ① SB-804HQ gel chromatographic column (7.8 mm×300 mm); ② mobile phase: 0.2 g·L-1 sodium azide solution, with flow-rate of 0.5 mL·min-1; ③ column temp.: 35 ℃; ④ vol. of sample introduced: 25 μL; ⑤ detection: 2410 differential refraction detector; ⑥ the glucan standard series of Shodex Standard P-82 with known Mr were chosen as calibration standards. It was found that the average Mr of β-glucan of Grifola frondosa (GF-932) mycelia were in the range 134 195-251 053 amu, and the breadth parameters (Mr/Mn) of Mr distribution were in the range of 1.64-2.61. It was shown that the analytes were polymeric substances of high-purity with definite range of distribution of Mr.