在酸性条件下,盐酸可卡因分子中氮原子被质子化后与阴离子AuCl4-形成离子缔合物,该缔合物被二氯甲烷带入含鲁米诺的氯化十六烷基三甲基铵反胶束微乳液中,离解出来的AuCl4-立即与鲁米诺产生化学发光,结果表明：发光强度与盐酸可卡因的质量浓度在0.001~10 mg·L-1范围内呈线性关系,据此提出了间接测定盐酸可卡因的微乳液介质-流动注射化学发光法。方法的检出限(3σ)为0.01 μg·L-1。以尿样和血清样品为基体,用标准加入法测定方法的回收率,测得回收率在95.0%~102.0%之间,相对标准偏差(n=5)在1.8%~3.2%之间。

It was found that in an acidic medium, an ion association complex was formed between AuCl4- and the protonated nitrogen atom of cocaine hydrochloride, and this complex was extracted with dichloromethane and the extract was transfered into a reversed micellar microemulsion prepared by mixing cetyltrimethylammonium chloride (CTAC) and luminol. Then AuCl4- was released and reacted with luminol to produce chemiluminescence. Based on these facts, a FI-CL method for indirect determination of cocaine hydrochloride was proposed. Linearity range of the working curve was kept between 0.001-10 mg·L-1, with detection limit (3σ) of 0.01 μg·L-1. Test for recovery was made by standard addition method using samples of urine and blood serum as matrixes, results of recovery found were in the range of 95.0%-102.0% with RSD′s (n=5) in the range of 1.8%-3.2%.