Determination of Glucose, Fructoes and Sucrose in Seabuckthorn Fruit Honey by HPLC with ELSD
摘 要
提出了高效液相色谱法测定沙棘果蜜中葡萄糖、果糖和蔗糖含量的方法。以Zorbax-NH2为色谱柱(4.6 mm×250 mm,5 μm),乙腈-水(90+10)混合溶液为流动相进行淋洗分离,用蒸发光散射检测器检测。峰面积的对数值与3种糖的质量浓度的对数值在10~1 000 mg·L-1范围内呈线性关系,方法的检出限(3S/N)均为10 mg·L-1。采用标准加入法做回收试验,平均回收率在95.0%~105.0%之间,相对标准偏差(n=5)均小于2.5%。
Abstract
HPLC was applied to the determinations of glucose, fructoes and sucrose in seabuckthorn fruit honey. The 3 saccharides were separated on Zorbax-NH2 (4.6 mm×250 mm, 5 μm) chromatographic column with a mixture of acetonitrile and water (90+10) as mobile phase, and their contents determined by evaporation light scattering detector (ELSD). Linear relationship between logarithmic values of peak area and mass concentration of the 3 saccharides were obtained in the same range of 10-1 000 mg·L-1 and with same detection limit (3S/N) of 10 mg·L-1. The values of average recovery found by standard addition method were in the range of 95.0%-105.0%, with values of RSD′s (n=5) less than 2.5%.
中图分类号 O652.63
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收稿日期 2010/7/13
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备注贾红卫(1967-),男,青海西宁人,高级工程师,主要从事食品安全研究。
引用该论文: JIA Hong-wei,LIN Tong,YAO Zhi-min,ZHAO Yu,GUAN Da-wei. Determination of Glucose, Fructoes and Sucrose in Seabuckthorn Fruit Honey by HPLC with ELSD[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2011, 47(9): 1066~1067
贾红卫,林童,姚志敏,赵玉,关大伟. 蒸发光散射检测-高效液相色谱法测定沙棘果蜜中的葡萄糖、果糖和蔗糖[J]. 理化检验-化学分册, 2011, 47(9): 1066~1067
被引情况:
【1】沈少林,陆文琪,张惠雄, "高效液相色谱-蒸发光散射法同时测定蜂蜜中果糖、葡萄糖、蔗糖和麦芽糖",理化检验-化学分册 51, 1330-1332(2015)
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参考文献
【1】SN/T 0852-2000进出口蜂蜜检验方法[S].
【2】张书芬,史萍萍,王全林,等.液相色谱示差折光法测定蜂蜜中的果糖、葡萄糖、蔗糖和麦芽糖[J].食品科学, 2008,29(6):280-283.
【3】丁建生.实用医学统计学[M].西安:陕西科技出版社, 1999:77-80.
【2】张书芬,史萍萍,王全林,等.液相色谱示差折光法测定蜂蜜中的果糖、葡萄糖、蔗糖和麦芽糖[J].食品科学, 2008,29(6):280-283.
【3】丁建生.实用医学统计学[M].西安:陕西科技出版社, 1999:77-80.
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