提出了纳米金在线富集-毛细管电泳法测定尿样中8-羟基-2′脱氧鸟嘌呤核苷(8-OHdG)的方法。试验选择了以下的分析条件:① 运行电压-20 kV;② 背景电解质为pH 8.2的20 mmol·L-1硼砂(含粒径10 nm纳米金溶液200 μL和0.1 mmol·L-1 CTMAB);③ 检测波长254 nm。试验证明背景电解质中的纳米金粒子与CTMAB形成胶束,提高了对8-OHdG的扫集能力,8-OHdG与dG在10 min内可实现基线分离。8-OHdG浓度在0.50~50.0 μmol·L-1范围内呈线性,检出限(3S/N)为39 nmol·L-1。方法用于尿样中8-OHdG含量的测定,所得加标回收率在90.0%~104.6%之间。

A method for the determination of 8-hydroxy-2′-deoxguanosine (8-OHdG) in urine sample by CE based on enrichment by online sweeping with nanogold was proposed. Analytical conditions were studied and following optimized parameters were given: ① moving voltage: -20 kV; ② electrophoretic medium: 20 mmol·L-1 of borax solution (pH 8.2, including 10 nm nanogold solution of 200 μL and 0.1 mmol·L-1 CTMAB; ③ wavelength of detection: 254 nm. It was shown that micelle was formed between nanogold and CTMAB in the electrophoretic medium, which conduced to the sweeping of 8-OHdG. The baseline resolution was attained within 10 min. Linearity range between 0.50 and 50.0 μmol·L-1 was obtained with detection limit (3S/N) of 39 nmol·L-1. The proposed method was used in the determination of 8-OHdG in a urine samples, gving recovery found by standard addition method in the range of 90.0%-104.6%.