用4种不同的样品前处理方法,即① 磷酸二氢钠萃取法(SE)、② 磷酸二氢钠萃取-固相萃取法(SE-SPE)、③ 基质固相分散萃取法(MSPDE)和④ 基质固相分散萃取-固相萃取法(MSPDE-SPE)对鸡肉样品做前处理,用高效毛细管电泳测定试液中青霉素、氨苄青霉素和阿莫西林等3种药物的残留量。回收试验和色谱分离结果表明:方法②用于高效毛细管电泳法同时测定鸡肉样品中3种青霉素类药物的样品前处理更为合适。方法的加标回收率在73.1%~95.9%之间,相对标准偏差(n=5)在2.9%~9.8%之间。

Chicken samples were pretreated by the following 4 methods separately: ① NaH2PO4-extraction (SE), ② NaH2PO4-solid phase extraction (SE-SPE), ③ matrix solid-phase dispersion extraction (MSPDE)and ④ matrix solid-phase dispersion extraction-SPE (MSPDE-SPE). The contents of penicillin, ampicillin and amoxicillin in the sample solutions were determined by high performance capillary electrophoresis (HPCE). As shown by the results obtained by recovery tests and chromatographic separation, the pretreatment method ② was more suitable to be used for simultaneous determination of the 3 penicillin drugs. Tests for recovery and precision of the proposed method were tested, values of recovery and RSD′s (n=5) found were in the ranges of 73.1%-95.9% and 2.9%-9.8% respectively.