用高效液相色谱法测定一种中成药“小儿热速清”口服液中的绿原酸、黄芩苷和葛根素含量。采用Zorbax SB-C18色谱柱分离,以不同比例混合的甲醇和乙酸-水(1+99)溶液为流动相,进行梯度洗脱,在327 nm波长处测定绿原酸、黄芩苷的含量,在250 nm波长处测定葛根素的含量。绿原酸质量浓度在3.0~30.0 mg·L-1范围内呈线性关系;黄芩苷质量浓度在2.7~27.0 mg·L-1范围内呈线性关系;葛根素质量浓度在3.1~31.0 mg·L-1范围内呈线性关系。方法的平均回收率为99.5%,99.8%,99.3%,相对标准偏差(n=6)分别为0.88%,0.84%,1.0%。

The effective components, i.e. chlorogenic acid, baicalin and puerarin, in a TCM preparation, named “xiao-er resuqing” oral liquor, were determined by HPLC. The Zorbax SB-C18 chromatographic column was used as the stationary phase and mixtures of methanol and CH3COOH-H2O (1+99) solution with various proportions were used in the gradient elution. UV detections at 327 nm (for chlorogenic aicd and baicalin) and at 250 nm (for puerarin) were adopted in the determinations. Linear relationships between values of peak area and mass concentration of the 3 compounds were found in the following ranges of ① 3.0-30.0 mg·L-1, ② 2.7-27.0 mg·L-1 and ③ 3.1-31.0 mg·L-1 respectively. Values of average recovery obtained were 99.5% (for chlorogenic acid), 99.8% (for baicalin) and 99.3% (for puerarin). Values of RSD′s (n=6) found for the 3 compounds were 0.88%, 0.84% and 1.0% respectively.