Inhibitory Catalytic Kinetic Photometric Determination of Trace Amount of EDTA in Canned Chestnuts
摘 要
在硫酸介质中,Fe(Ⅲ)对过氧化氢氧化中性红的褪色反应具有强的催化作用,EDTA对上述指示反应具有灵敏的阻抑作用,从而提出了一种阻抑催化动力学光度法测定板栗罐头中痕量EDTA的方法。优化的试验条件如下:① 过氧化氢用量为1.0 mL;② 0.1 g·L-1中性红溶液用量1.0 mL;③ 50.0 mg·L-1 Fe(Ⅲ)溶液用量为0.12 mL;④ 反应温度为沸水浴;⑤ 反应时间为10 min。EDTA的质量浓度在900 μg·L-1以内与ΔA呈线性关系,检出限(3s/k)为8 μg·L-1。方法用于板栗罐头中EDTA的测定,测定值的相对标准偏差(n=6)小于3.0%,加标回收率为93.0%~96.0%。
Abstract
It was found that in H2SO4 medium, the oxidation of neutral red by hydrogen peroxide was strongly catalyzed by Fe(Ⅲ), leading to an enhancement of color fading of neutral red. This indicative reaction was inhibited by the presence of EDTA. Based on these facts, the determination of traces of EDTA in canned chestnuts by inhibitory catalytic kinetic photometry was proposed. The optimized conditions found were as follows: ① amount of hydrogen peroxide solution: 1.0 mL; ② amount of 0.1 g·L-1 neutral red solution: 1.0 mL; ③ amount of 50.0 mg·L-1 Fe(Ⅲ) solution: 0.12 mL; ④ temperature of reaction: boiling water bath; ⑤ time of reaction: 10 min. Linear relationship between values of ΔA and mass concentration of EDTA was obtained in the range within 900 μg·L-1. Detection limit (3s/k) of the method found was 8 μg·L-1. The proposed method was applied to the determination of EDTA in canned chestnuts. Recovery was tested by addition of standard solution to the chestnut sample, giving values of recovery ranged from 93.0%-96.0%, and values of RSD (n=6) less than 3.0%.
中图分类号 O657.32
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收稿日期 2011/2/4
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备注陈伟(1978-),男,山东临沂人,主管检验技师,硕士,研究方向为光谱分析。
引用该论文: CHEN Wei,JIA Feng-yan,WANG Xiang-min,WU Xu-ran,LIU Yong-ming. Inhibitory Catalytic Kinetic Photometric Determination of Trace Amount of EDTA in Canned Chestnuts[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(1): 15~17
陈伟,贾风燕,王湘敏,邬旭然,刘永明. 阻抑催化动力学光度法测定板栗罐头中痕量EDTA[J]. 理化检验-化学分册, 2012, 48(1): 15~17
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参考文献
【1】王琼,方艳红.罐头食品中EDTA残留量的薄层色谱测定[J].华侨大学学报:自然科学版, 2002,23(1):37-39.
【2】BELAL F, ALY F, WALASH M, et al. Determination of ethylenediaminetetraacetic acid (EDTA) in pharmaceutical dosage forms using flame atomic absorption spectroscopy[J]. Il Farmaco, 1998,53(5):365-368.
【3】BERGERS P, DE GROOT A. The analysis of EDTA in water by HPLC[J]. Water Research, 1994,28(3):639-642.
【4】KORD A, TUMANOVA I, MATIER W. A novel HPLC method for determination of EDTA in a cataract inhibiting ophthalmic drug[J]. Journal of Pharmaceutical and Biomedical Analysis, 1995,13(4/5):575-580.
【5】BHATTACHARYYA S, KUNDU K. Spectrophotometric determination of EDTA[J]. Talanta, 1971,18(4):446.
【6】KVASNI KA F, MKOV K. Determination of EDTA in mayonnaise by on-line coupled capillary isotachophoresis-capillary zone electrophoresis with UV detection[J]. Journal of Food Composition and Analysis, 1996,9(3):231-242.
【7】BUERGISSER C, STONE A. Determination of EDTA, NTA, and other amino carboxylic acids and their Co(Ⅱ) and Co(Ⅲ) complexes by capillary electrophoresis[J]. Environmental Science & Technology, 1997,31(9):2656-2664.
【8】POZDNIAKOVA S, RAGAUSKAS R, DIK I A, et al. Determination of EDTA in used fixing solutions by capillary electrophoresis[J]. Fresenius′ Journal of Analytical Chemistry, 1999,363(1):124-125.
【9】FINGERHUT R, DAME T, OLGEM L B. Determination of EDTA in dried blood samples by tandem mass spectrometry avoids serious errors in newborn screening[J]. European Journal of Pediatrics, 2009,168(5):553-558.
【10】陈伟.阻抑动力学光度法间接测定痕量EDTA[J].分析试验室, 2006(1):87-89.
【11】陈伟,陈惠,李英.铁(Ⅲ)-H2O2-溴百里酚蓝体系阻抑动力学光度法测定痕量EDTA[J].理化检验-化学分册, 2007,43(8):690-691.
【12】刘海玲,谢瑞容.动力学光度法测定微量乙二胺四乙酸[J].分析化学, 2000,28(8):1050-1050.
【13】陈国树,阳小燕.环境水样中邻苯二胺测定的阻抑动力学光度法[J].分析测试学报, 2000,19(6):21-24.
【2】BELAL F, ALY F, WALASH M, et al. Determination of ethylenediaminetetraacetic acid (EDTA) in pharmaceutical dosage forms using flame atomic absorption spectroscopy[J]. Il Farmaco, 1998,53(5):365-368.
【3】BERGERS P, DE GROOT A. The analysis of EDTA in water by HPLC[J]. Water Research, 1994,28(3):639-642.
【4】KORD A, TUMANOVA I, MATIER W. A novel HPLC method for determination of EDTA in a cataract inhibiting ophthalmic drug[J]. Journal of Pharmaceutical and Biomedical Analysis, 1995,13(4/5):575-580.
【5】BHATTACHARYYA S, KUNDU K. Spectrophotometric determination of EDTA[J]. Talanta, 1971,18(4):446.
【6】KVASNI KA F, MKOV K. Determination of EDTA in mayonnaise by on-line coupled capillary isotachophoresis-capillary zone electrophoresis with UV detection[J]. Journal of Food Composition and Analysis, 1996,9(3):231-242.
【7】BUERGISSER C, STONE A. Determination of EDTA, NTA, and other amino carboxylic acids and their Co(Ⅱ) and Co(Ⅲ) complexes by capillary electrophoresis[J]. Environmental Science & Technology, 1997,31(9):2656-2664.
【8】POZDNIAKOVA S, RAGAUSKAS R, DIK I A, et al. Determination of EDTA in used fixing solutions by capillary electrophoresis[J]. Fresenius′ Journal of Analytical Chemistry, 1999,363(1):124-125.
【9】FINGERHUT R, DAME T, OLGEM L B. Determination of EDTA in dried blood samples by tandem mass spectrometry avoids serious errors in newborn screening[J]. European Journal of Pediatrics, 2009,168(5):553-558.
【10】陈伟.阻抑动力学光度法间接测定痕量EDTA[J].分析试验室, 2006(1):87-89.
【11】陈伟,陈惠,李英.铁(Ⅲ)-H2O2-溴百里酚蓝体系阻抑动力学光度法测定痕量EDTA[J].理化检验-化学分册, 2007,43(8):690-691.
【12】刘海玲,谢瑞容.动力学光度法测定微量乙二胺四乙酸[J].分析化学, 2000,28(8):1050-1050.
【13】陈国树,阳小燕.环境水样中邻苯二胺测定的阻抑动力学光度法[J].分析测试学报, 2000,19(6):21-24.
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