提出了高效液相色谱法测定平胃丸中橙皮苷含量的方法。采用Hypersil BDS C18(200 mm×4.6 mm,5 μm)色谱柱;以甲醇-乙酸-水(35+4+61)混合溶液为流动相,流量1 mL·min-1,检测波长为283 nm,橙皮苷的质量浓度与峰面积在8.08~121.2 mg·L-1范围内呈线性关系。方法用于平胃丸中橙皮苷的测定,平均回收率为101.1%,相对标准偏差(n=9)为1.2%。

HPLC was applied to the determination of hesperidin in the prepared tradition Chinese medicine named “Pingwei Wan”. The Hypersil BDS C18 column (250 mm×4.6 mm, 5 μm) was used as stationary phase, and a mixture of methanol, acetic and water, mixed in the ratio of 35 to 4 to 61 by volume, was used as mobile phase with a flow-rate of 1 mL·min-1. Detection at the wavelength of 283 nm was adopted in the determination. Linear relationship between values of peak area and mass concentration of hesperidin was obtained in the range of 8.08-121.2 mg·L-1. The proposed method was used in the determination of hesperidin in the drug, giving average recovery of 101.1% with RSD (n=9) 1.2%.