利用衰减全反射傅里叶红外光谱法对三种掺假蜂蜜进行了快速鉴别。对掺入的蔗糖、葡萄糖的蜂蜜的特征吸收峰进行了多峰位的比较,判定是否为掺假蜂蜜。对掺入不同含量果葡糖浆的蜂蜜红外图谱,进行计算软件处理后通过二阶导数图谱在1 054 cm-1、817 cm-1两处的吸收峰,可以准确的判定掺入蜂蜜中果葡糖浆的含量。对掺入蜂蜜中的三种物质蔗糖、葡萄糖、果葡糖浆的最小检出限量为10%。该方法样品用量少、操作简便、无需前处理、分析速度快,可作为市场筛查掺假蜂蜜的快速检测方法。

FT-IRS with attenuated total reflection (ATR) was applied to the rapid discrimination of adulteration of honey. Characteristic absorption peaks of honey mixed with various amounts of sucrose or glucose were compared and on the base of the difference in absorption peaks, adulteration of honey was determined. As treating the IR spectra of samples of honey mixed with various amounts of high fructose corn syrup (HFCS) by the calculation software, two absorption peaks at 1 054 cm-1 and 817 cm-1 shown on the 2nd derivative spectra obtained were taken as the basis to judge accurately the amount of HFCS admixed. Values of detection limit of 3 admixtures, i.e., sucrose, glucose and HFCS were found same as 10%. The proposed method has the special features of requring less sample, simple and easy in operation, speedy analysis and feasible for use in large-scale randon checking and supervision.