Preparation of MWCNT′s-SH/AuNP′s/ME and Its Application to Determination of Dopamine
摘 要
通过羧基化多壁碳纳米管(MWCNT′s-COOH)与巯基乙胺反应制得巯基化多壁碳纳米管(MWCNT′s-SH),将MWCNT′s-SH加入含有硼氢化钠的氯金酸溶液中,使生成的纳米金颗粒(AuNP′s)自组装到MWCNT′s-SH上,制得MWCNT′s-SH/AuNP′s粉末。将MWCNT′s-SH/AuNP′s粉末与微电极(ME)反复磨压使粉末进入ME顶端小孔内,烘干后即得MWCNT′s-SH/AuNP′s/ME。采用循环伏安法研究了多巴胺在修饰电极上的电化学行为。结果表明:多巴胺在该修饰电极上出现了一对氧化还原峰。多巴胺线性范围为5.0×10-6~6.4×10-4mol·L-1,检出限(3S/N)为5.1×10-9mol·L-1。修饰电极用于盐酸多巴胺注射液中多巴胺的测定,测定值与标示值相符,加标回收率在98.0%~103.0%之间。
Abstract
Sulfhydryl multiwalled carbon nanotubes (MWCNT′s-SH) were prepared by the reaction of carboxy multiwalled carbon nanotubes (MWCNT′s-COOH) with sulfhydryl ethylamine. The MWCNT′s-SH was then added to a reaction mixture containing NaBH4 and HAuCl4, by which the gold nanoparticles (AuNP′s) produced were self-assembled onto MWCNT′s-SH and MWCNT′s-SH/AuNP′s powder was obtained. By pulverization and pressing of the MWCNT′s-SH/AuNP′s powder with the microelectrode (ME) to have the powder filled into the small hole at the top of ME, the modified MWCNT′s-SH/AuNP′s/ME was prepared. Electrochemical behavior of the modified electrode was studied by cyclic voltammetry. It was found that a pair of redox peaks given by dopamine was observed at the modified electrode. Linearity range for dopamine between 5.0×10-6 and 6.4×10-4mol·L-1 was obtained, with detection limit (3S/N) of 5.1×10-9mol·L-1. The proposed method was used in the determination of dopamine in injection samples, giving results in consistency with the labeled values. Recovery of the method was tested by standard addition method, giving recovery in the range of 98.0%-103.0%.
中图分类号 O657.1
所属栏目 试验与研究
基金项目 国家自然科学基金资助项目(No.21101024);教育部科学技术研究重点研究项目(No.211086)
收稿日期 2011/5/23
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联系人作者张明明(xhcao@ecit.cn)
备注张明明(1986-),男,安徽人,硕士研究生,主要研究方向为电分析化学。
引用该论文: ZHANG Ming-ming,CAO Xiao-hong,HUANG Ping-di. Preparation of MWCNT′s-SH/AuNP′s/ME and Its Application to Determination of Dopamine[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(3): 253~256
张明明,曹小红,黄平弟. 自组装纳米金的多壁碳纳米管微电极的制备及其在测定多巴胺中的应用[J]. 理化检验-化学分册, 2012, 48(3): 253~256
被引情况:
【1】张钱丽,肖昕,张蓉,戴鹏,周帅,冯刚, "介孔碳/纳米金修饰电极同时测定多巴胺、抗坏血酸和尿酸",理化检验-化学分册 51, 1018-1022(2015)
【2】朱旭,吴淑芳,李春兰,徐茂田,冶保献, "聚L-精氨酸/纳米金修饰电极测定多巴胺",理化检验-化学分册 51, 1057-1062(2015)
【3】努尔古再丽·艾则孜,姜 娜,李桂新, "用鲁米诺功能化金纳米粒子修饰的金电极电化学发光法测定多巴胺",理化检验-化学分册 52, 378-383(2016)
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【3】孙登明,马伟,张振新.聚L-赖氨酸修饰电极循环伏安法测定药剂中的多巴胺[J].分析化学, 2006,34(5):668-670.
【4】CHEN R S, HUANG W H, HUA TONG Z L W, et al. Carbon fiber nanoelectrodes modified by single-walled carbon nanotubes[J]. Anal Chem, 2003,75(22):6341-6345.
【5】VIVIERA V, RGISB A, SAGONB G, et al. Cyclic voltammetry study of bismuth oxide Bi2O3 powder by means of a cavity microelectrode coupled with raman microspectrometry[J]. Electrochim Acta, 2001,46(6):907-914.
【6】HENRY C S, FRITSCH I. Microfabricated recessed microdisk electrodes: characterization in static and convective solutions[J]. Anal Chem, 1998,71(3):550-556.
【7】FENG Xiu-mei, HU Jian-qiang, CHEN Xiao-hua, et al. Synthesis and electron transfer property of sulfhydryl-containing multi-walled carbon nanotube/gold nanoparticle heterojunctions[J]. J Phys D: Appl Phys, 2009,42(4):1-6.
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