无泵负压进样-芯片电泳分离-激光诱导荧光光谱法测定儿茶酚胺类物质
Separation and Determination of Catecholamines by Microchip Electrophoresis with Laser Induced Fluorescence Spectrometry and Pump-free Negative Pressure Sample Introduction
摘 要
提出了用芯片电泳分离-激光诱导荧光光谱法测定儿茶酚胺类物质的方法。采用自制的无泵负压进样系统,避免了进样歧视效应。在优化的条件下,去甲肾上腺素(NE)、多巴胺(DA)和肾上腺素(E)可在1 min内完全分离。3种儿茶酚胺的平均迁移时间依次为30.59,37.23,46.43 s,其相对标准偏差(n=7)依次为1.10%,1.28%,0.45%。3种物质的线性范围为0.3~5.0 mg·L-1 (NE及DA)和0.05~4.0 mg·L-1 (E),检出限(3S/N)依次为30,30,10 μg·L-1。
激光诱导荧光光谱法
儿茶酚胺类物质
Microchip electrophoresis
Laser induced fluorescence spectrometry
Catecholamines
Abstract
The method of microchip electrophoresis with laser induced fluorescence detection was proposed to be used in the separation and determination of catecholamines, including norepinephrine (NE), dopamine (DA) and epinephrine (E). The application of a self-made pump-free negative pressure sample introduction system made it possible to avoid the discrimination effect in sample introduction. NE, DA and E were separated completely within 1 min under the optimum working conditions, with their average migration times of 30.59, 37.23, 46.43 s and values of RSD′s (n=7) of 1.10%, 1.28%, 0.45% respectively. Linearity ranges found were 0.3-5.0 mg·L-1 (for NE and DA) and 0.05-4.0 mg·L-1 (for E), with detection limits (3S/N) of 30, 30, 10 μg·L-1 respectively.
中图分类号 O657.3
所属栏目 试验与研究
基金项目 广东省自然科学基金项目(S2011040002246)
收稿日期 2011/8/1
修改稿日期
网络出版日期
作者单位点击查看
联系人作者胡红美(huhm@zju.edu.cn)
备注胡红美(1986-),女,浙江衢州人,硕士,主要从事微流控芯片和气相色谱分析。
引用该论文: HU Hong-mei,WANG Xiu-zhong,XU Chun-xiu,YIN Xue-feng,SUN Xiu-mei,GUO Yuan-ming. Separation and Determination of Catecholamines by Microchip Electrophoresis with Laser Induced Fluorescence Spectrometry and Pump-free Negative Pressure Sample Introduction[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(4): 377～380
胡红美,王修中,徐春秀,殷学锋,孙秀梅,郭远明. 无泵负压进样-芯片电泳分离-激光诱导荧光光谱法测定儿茶酚胺类物质[J]. 理化检验－化学分册, 2012, 48(4): 377～380
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【3】ZHANG Lei, YIN Xue-feng, FANG Zhao-lun. Negative pressure pinched sample injection for microchip-based electrophoresis［J］. Lab on a Chip, 2006,6(2):258-264．
【4】ZHANG Lei, YIN Xue-feng. Parallel separation of multiple samples with negative pressure sample injection on a 3-D microfluidic array chip［J］. Electrophoresis, 2007,28(8):1281-1288．
【5】HU Hong-mei, YIN Xue-feng, QI Li-ya, et al. Pump-free and low-cost negative pressure sampling device for rapid sample loading in MCE［J］. Electrophoresis, 2009,30(24):4213-4218．
【6】ZHOU Lei, WANG Shu-min, TIAN Kan, et al. Simultaneous determination of catecholamines and amino acids by micellar electrokinetic chromatography with laser-induced fluorescence detection［J］. Journal of Separation Science, 2007,30(1):110-117．
【7】ZHOU Lei, WANG Wei-ping, WANG Shu-min, et al. Nonaqueous capillary electrophoresis with laser-induced fluorescence detection: A case study of comparison with aqueous media［J］. Anal Chim Acta, 2008,611(2):212-219．
【8】LING Yun-yang, YIN Xue-feng, FANG Zhao-lun. Simultaneous determination of glutathione and reactive oxygen species in individual cells by microchip electrophoresis［J］. Electrophoresis, 2005,26(24):4759-4766．
【9】MOLINA M, SILVA M. Micellar electrokinetic chromatography: Current developments and future［J］. Electrophoresis, 2002,23(22/23):3907-3921．
【10】MARSH A, CLARK B, BRODERICK M, et al. Recent advances in microemulsion electrokinetic chromatography［J］. Electrophoresis, 2004,25(23/24):3970-3980．
【11】PAPPAST J, GAYTON-ELY M, HOLLAND L A. Recent advances in micellar electrokinetic chromatography［J］. Electrophoresis, 2005,26(4/5):719-734．
【12】CHEN Nong, TERABE S, NAKAGAWA T. Effect of organic modifier concentrations on electrokinetic migrations in micellar electrokinetic chromatography［J］. Electrophoresis, 1995,16(1):1457-1462．
【13】HUIE C W. Effects of organic solvents on sample pretreatment and separation performances in capillary electrophoresis［J］. Electrophoresis, 2003,24(10):1508-1529．
【14】RODRIGUEZ E M R, ALAEJOSM S, ROMERO C D. Enhancement of the fluorescence intensity of Se-2,3-diaminonaphthalene complex in aqueous solution by adding organic solvents［J］. Anal Chim Acta, 1996,334(1/2):161-166．
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