应用响应面法优化了乙醇微波提取板栗花中总黄酮的工艺条件。用光度法在波长510 nm处测定提取液中总黄酮的含量(以芦丁为标准),从而判断提取条件的优劣。经试验确定最优提取条件为:① 微波功率700 W;② 提取时间80 s;③ 提取溶剂乙醇与水的体积比为40比60;④ 板栗花料与提取溶剂的比值为1比100。在此选定条件下板栗花总黄酮的提取率达到(3.47±0.14) g/100 g,与理论值(3.49 g/100 g)很接近。

The technological conditions for microwave assisted extraction of total flavonoids in chestnut flower with ethanol were optimized by applying the method of response surface. Contents of total flavonoids in the extracts were determined photometrically at the wavelength of 510 nm (using rutin as reference standard) to decide which of the conditions is better or worse. The optimum conditions found for the extraction were as follws: ① power of microwave 700 W; ② time of extraction 80 s; ③ concentration of the extractant (ehanol) C2H5OH-H2O (40+60); ④ mass ratio of the raw material (chestnut flower) to extractant (ethanol) 1∶100. Rate of extraction obtained under the optimum conditions attained to (3.47±0.14) g/100 g, which is very close to the theoratical value (3.49 g/100 g).