提出了高效液相色谱法同时测定牛膝配方颗粒中5-羟甲基糠醛和蜕皮甾酮的含量的方法。采用SHIMADZU shim-pack VP-ODS(4.6 mm ×250 mm,4.6 μm)柱;用两种不同配比的乙腈和水混合溶液作为流动相,梯度洗脱;检测波长为279 nm(5-羟甲基糠醛)和245 nm(蜕皮甾酮)。5-羟甲基糠醛和蜕皮甾酮的质量浓度分别在0.390~100 mg·L-1和3.125~100 mg·L-1范围内与峰面积呈线性关系,方法的检出限(3S/N)分别为1.95, 2.01 ng。5-羟甲基糠醛和蜕皮甾酮的平均回收率分别为99.7%,99.1%;相对标准偏差(n=5)分别为0.80%,0.67%。

HPLC was applied to the simultaneous determination of 5-hydroxymethylfurfural and ecdysterone in granules of radix achyranthis bientatae for making up prescriptions. SHIMADZU shim-pack VP-ODS column (4.6 mm×250 mm, 4.6 μm) was used for separation. Mixtures containing acetonitrile and water mixed in different ratio were used as the mobile phase in gradient elution. UV-detection at the wavelengths of 279 nm (for 5-hydroxymethylfurfural) and 245 nm (for ecdysterone) was adopted in the determination. Linear relationships between values of peak area and mass concentration of 5-hydroxymethylfurfural and ecdysterone were obtained in the ranges of 0.390-100 mg·L-1 and 3.125-100 mg·L-1, with detection limits (3S/N) of 1.95 ng and 2.01 ng respectively. Results of recovery and precision found were: values of average recovery of 99.7% for 5-hydroxymethylfurfural and 99.1% for ecdysteronen with values of RSD′s (n=5) 0.80% and 0.67% respectively.