基于纳米银-曙红Y-DNA的荧光共振能量转移探针
Fluorescence Resonance Energy Transfer Probe Based on Nano-Ag-EY-DNA System
摘 要
以曙红Y-DNA为荧光探针,运用紫外可见光谱法、荧光光谱法和电泳法研究了纳米银与曙红Y-DNA之间的共振能量转移,并应用能量共振转移的原理解释了纳米银及曙红Y与DNA之间的相互作用。结果表明,曙红Y与DNA的作用方式为嵌插作用,纳米银与DNA的作用方式为静电作用,曙红Y和纳米银结合到DNA上以后,导致了DNA结构的改变。
DNA
曙红Y
荧光共振能量转移
探针
Nano-Ag
DNA
Eosin Y
Fluorescence resonance energy transfer
Probe
Abstract
The resonance energy transfer between nano-Ag and eosin Y (EY)-DNA was studied by UV-Vis spectrophotometry, fluorospectrophotometry and electrophoresis with EY-DNA as fluorescence probe. The phenomenon of interaction between nano-Ag and EY-DNA was explained by the theory of fluorescence resonance energy transfer (FRET). It was found that the reaction of EY with DNA was due to inserting action, and the reaction between nano-Ag and DNA was due to electrostatic action. The structure of DNA was changed after binding with EY and nano-Ag.
中图分类号 O657.3
所属栏目 试验与研究
基金项目 山东省自然科学基金(ZR2010BL026和ZR2011BL026)与山东省高校科技计划(J09LC66)共同资助项目
收稿日期 2011/7/21
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备注魏光成(1977-),男,山东淄博人,硕士,讲师,研究方向为光分析化学。
引用该论文: WEI Guang-cheng,YAN Miao-miao,MA Li-ying. Fluorescence Resonance Energy Transfer Probe Based on Nano-Ag-EY-DNA System[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(6): 643～645
魏光成,闫苗苗,马丽英. 基于纳米银-曙红Y-DNA的荧光共振能量转移探针[J]. 理化检验－化学分册, 2012, 48(6): 643～645
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参考文献
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【2】王运庆,叶超,王晓梅,等.荧光量子点在纳米药物研究中的应用进展［J］.中国药科大学学报, 2009,40(1):8-15.
【3】ZHOU D J, JOE D P, CHRIS A, et al. Fluorescence resonance energy transfer between a quantum dot donor and a dye acceptor attached to DNA［J］. Chem Commun, 2005,38:4807-4809.
【4】RUMIANA B, ZHIVKO Z, HIDEKI O, et al. Quantum dot-conjugated hybridization probes for preliminary screening of siRNA sequences［J］. J Am Chem Soc, 2005,127(32):11328-11335.
【5】CHARUDHARSHINI S, JEUNGHOON, L, FOTIOS P, et al. Labeling and intracellular tracking of functionally active plasmid DNA with semiconductor quantum dots［J］. Mol Ther, 2006,14(2):192-201.
【6】吴峰,蔡继业.基于量子点的荧光共振能量转移的应用［J］.化学与生物工程, 2007,24(1):58-61.
【7】HO Y P, CHEN H H, LEONG K W, et al. Evaluating the intracellular stability and unpacking of DNA nanocomplexes by quantum dots-FRET［J］. J Control Release, 2006,116(1):83-89.
【8】ALGAR W R, ULRICH J K. Quantum dots as donors in fluorescence resonance energy transfer for the bioanalysis of nucleic acids, proteins, and other biological molecules［J］. Anal Bioanal Chem, 2008,391:1609-1611.
【9】RON G, MAYA Z, ITAMAR W. Semiconductor quantum dots for bioanalysis［J］. Angew Chem Int Ed, 2008,47(40):7602-7625.
【10】孙墨杰,贾若琨,张宇帅,等.纳米银的制备研究［J］.东北电力大学学报, 2008,28(1):5-7.
【11】杨昌英,刘义.两种荧光探针法研究三种非甾体抗炎药与DNA的相互作用［J］.化学学报, 2007,65(18):2076-2080.
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