提出了用同步荧光测定法同时测定盐酸普萘洛尔和盐酸氟桂利嗪。试验表明:荧光检测盐酸普萘洛尔的波长宜选定296 nm、盐酸氟桂利嗪的波长宜选定263 nm、波长差Δλ为50 nm条件下进行同步扫描。盐酸普萘洛尔和盐酸氟桂利嗪的质量浓度分别在1.2×10-6~2.8×10-3g·L-1和2.0×10-5~3.6×10-3g·L-1范围内与荧光强度呈线性关系,检出限(3S/N)分别为3.2×10-7g·L-1和6.8×10-6g·L-1。方法用于混合样品中盐酸普萘洛尔与盐酸氟桂利嗪含量的同时测定,回收率在97.5%~101.1%和97.5%~101.7%之间。

Synchronous fluorimetry was proposed for the simultaneous determination of propranolol hydrochloride and flunarizine hydrochloride. It was found that satisfactory results were obtained by setting at the wavelength 296 nm for determination of propranolol hydrochloride, 263 nm for flunarizine hydrochloride, and wavelength difference (Δλ) 50 nm in the synchronous fluorescence measurements. Linear relationships between values of fluorescence intensity and mass concentration were found in the ranges of 1.2×10-6-2.8×10-3g·L-1 for propranolol hydrochloride and 2.0×10-5-3.6×10-3g·L-1 for and flunarizine hydrochloride, with detection limits (3S/N) of 3.2×10-7 and 6.8×10-6g·L-1 respectively. The proposed method was used for determination of propranolol hydrochloride and flunarizine hydrochloride in mixed samples, giving values of recovery in the ranges of 97.5%-101.1% for propranolol hydrochloride and 97.5%-101.7% for flunarizine hydrochloride.