Fluorophotometric Study on the Formation of Inclusion Complex Between Oleanic Acid and β-Cyclodextrin
摘 要
在磷酸、硼酸及乙酸组成的缓冲介质中,齐墩果酸与β-环糊精反应生成包合络合物,其包合摩尔比为1比1。包合物的激发波长及发射波长的峰分别位于355 nm及404 nm处,在25 ℃条件下的结合常数为71 L·mol-1,还测定了反应的热力学参数。测得ΔH0与ΔS0均大于0,说明此包合反应为吸热反应。测得ΔG0值小于0,且随反应温度升高而变得更负,说明此反应在试验条件下能自发进行,而随反应温度的升高,反应的自发性增强。
Abstract
It was found that in a buffer solution of H3PO4-H3BO3-HOAc, an inclusion complex was formed between oleanic acid (OLA) and β-cyclodextrin (β-CD) with mole ratio of 1 to 1. Excitation and emission maxima of the complex were found at wavelengths of 355 nm and 404 nm respectively. Value of association constant found at 25 ℃ was 71 L·mol-1, thermodynamic parameters of the complex were also determined. Values of ΔH0 and ΔS0 were both shown to be greater than 0, which proved that the inclusion reaction was endothermic. Value of ΔG0 was found to be smaller than 0, and becoming smaller as the reaction temperature increases, which proved that the inclusion reaction proceeds spontaneously and that the spontaneity of the reaction increases with the raise of reaction temperature.
中图分类号 O657.32
所属栏目 试验与研究
基金项目 河北省科技支撑计划项目(09276438)
收稿日期 2011/5/17
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备注王伟(1972-),女,河北新河人,高级实验师,主要从事药物分析研究。
引用该论文: WANG Wei,WANG Jing,YANG Cai-qin,SUN Yan,Lv Ya-qi,DONG Li-long. Fluorophotometric Study on the Formation of Inclusion Complex Between Oleanic Acid and β-Cyclodextrin[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(7): 760~762
王伟,王静,杨彩琴,孙严,吕雅琪,董立龙. 荧光光谱法研究β-环糊精与齐墩果酸生成的包合物[J]. 理化检验-化学分册, 2012, 48(7): 760~762
被引情况:
【1】翁文婷,张春, "基于三聚氰胺-铜(Ⅱ)-牛血清蛋白络合物的荧光光度法测定饲料中三聚氰胺",理化检验-化学分册 49, 1489-1492(2013)
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参考文献
【1】邹盛勤,罗小凤.HPLC-PAD法测定齐墩果酸原料及片剂中的齐墩果酸含量[J].安徽医药, 2005,9(11):826-827.
【2】邹美如,陈茶梅,付强.薄层扫描法测定参鹿补片中齐墩果酸的含量[J].江西中医学院学报, 2003,15(20):58-60.
【3】李必波,赵领,张雪梅,等.紫外光谱法测定齐墩果酸缓释滴丸药物含量及体外释放度[J].重庆医科大学学报, 2009,34(4):447-451.
【4】魏丹毅,葛从辛,周湘池,等.β-环糊精及其衍生物对Al(Ⅲ)-铁试剂的荧光增强效应及机理[J].化学研究与应用, 2000,12(6):626-628.
【5】李满秀,赵二劳,薛圆圆.β-环糊精增敏荧光法测定氯氰菊酯的研究[J].分析科学学报, 2007,23(5):592-594.
【6】BERZAS N J J, MURILLO P J A, GOMEZ L M A. Spectrofluorimetric study of the β-cyclodextrin:vitamin K3 complex and determination of vitamin[J]. Talanta, 2001,53(5):951-959.
【7】续浩,陈亮.环糊精包结物包结比的测定[J].分析测试技术与仪器, 2001,7(3):152-155.
【8】张海容,王文艳,陈金娥,等.荧光法研究萘酚与胍的包合性质[J].化学研究与应用, 2007,19(10):1153-1157.
【2】邹美如,陈茶梅,付强.薄层扫描法测定参鹿补片中齐墩果酸的含量[J].江西中医学院学报, 2003,15(20):58-60.
【3】李必波,赵领,张雪梅,等.紫外光谱法测定齐墩果酸缓释滴丸药物含量及体外释放度[J].重庆医科大学学报, 2009,34(4):447-451.
【4】魏丹毅,葛从辛,周湘池,等.β-环糊精及其衍生物对Al(Ⅲ)-铁试剂的荧光增强效应及机理[J].化学研究与应用, 2000,12(6):626-628.
【5】李满秀,赵二劳,薛圆圆.β-环糊精增敏荧光法测定氯氰菊酯的研究[J].分析科学学报, 2007,23(5):592-594.
【6】BERZAS N J J, MURILLO P J A, GOMEZ L M A. Spectrofluorimetric study of the β-cyclodextrin:vitamin K3 complex and determination of vitamin[J]. Talanta, 2001,53(5):951-959.
【7】续浩,陈亮.环糊精包结物包结比的测定[J].分析测试技术与仪器, 2001,7(3):152-155.
【8】张海容,王文艳,陈金娥,等.荧光法研究萘酚与胍的包合性质[J].化学研究与应用, 2007,19(10):1153-1157.
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