磷酸盐活化玻碳电极应用于肾上腺素的伏安测定
Use of Phosphate Activated Glassy Carbon Electrode in Voltammetric Determination of Epinephrine
摘 要
采用循环伏安法制备了磷酸盐活化玻碳电极,研究了在pH 6.0磷酸盐缓冲介质中肾上腺素在磷酸盐活化玻碳电极上的电化学行为,建立了循环伏安法测定痕量肾上腺素的方法,该方法可有效地排除抗坏血酸对肾上腺素的干扰。在电位值为0.26 V(vs.SCE)处,肾上腺素在磷酸活化玻碳电极上有一灵敏的氧化峰。肾上腺素浓度cEP在1.0×10-6~1.2×10-5mol·L-1范围内与氧化峰电流ipa呈线性关系。检出限(3S/N)为3.0×10-7mol·L-1。方法应用于肾上腺素注射液的分析,测得肾上腺素含量与标示值相符。用标准加入法测得方法的回收率为99.7%。
肾上腺素
磷酸盐
玻碳电极
电化学活化
Cyclic voltammetry
Epinephrine
Phosphate
Glassy carbon electrode
Electrochemical activation
Abstract
Phosphate activated GCE was prepared by cyclic voltammetry, and the electrochemical behavior of epinephrine (EP) at this electrode in a phosphate buffer medium of pH 6.0 was studied. A cyclic voltammetric method for determination of trace amount of EP was proposed, by which the interference of ascorbic acid to the determination of EP was effectively eliminated. A sensitive oxidation peak of EP at 0.26 V (vs.SCE) was observed. Linear relationship between values of oxidation peak current (ipa) and concentration of EP was obtained in the range of 1.0×10-6-1.2×10-5mol·L-1, with detection limit (3S/N) of 3.0×10-7mol·L-1. This method was applied to the determination of EP in injections, giving results in consistency with the labelled values. Average recovery of the method found by standard addition method was 99.7%.
中图分类号 O657.1
所属栏目 工作简报
基金项目 国家自然科学基金(21105023);中国博士后科学基金(200902558;20080430193);山东省自然科学基金(ZR2009BM003)
收稿日期 2011/5/11
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联系人作者李金金(yuzywxl@163.com)
备注李金金(1984-),女,山东济宁人,硕士研究生,研究方向为生命电分析化学。
引用该论文: LI Jin-jin,WANG Xue-liang,GUO Xian-hou,YU Zhang-yu. Use of Phosphate Activated Glassy Carbon Electrode in Voltammetric Determination of Epinephrine[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(7): 766～769
李金金,王学亮,郭宪厚,郁章玉. 磷酸盐活化玻碳电极应用于肾上腺素的伏安测定[J]. 理化检验－化学分册, 2012, 48(7): 766～769
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参考文献
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【2】FOTOPOULOU M A, LOANNOU P C. Post-column terbium complexation and sensitized fluorescence detection for the determination of norepinephrine, epinephrine and dopamine using high-performance liquid chromatography［J］. Anal Chim Acta, 2002,462(2):179-185.
【3】SHELKOVNIKOV S, GONICK H C. Peroxynitrite but not nitric oxide donors destroys epinephrine: HPLC measurement and rat aorta contractility［J］. Life Sci, 2004,75(23):2765-2773.
【4】童裳伦,朱岩.稀土离子荧光探针对肾上腺素的测定［J］.分析化学, 2000,28(3):293-295.
【5】KANG Wei-jun, NIU Ling-mei, MA Li. 2,3-Dimercaptosuccinic acid self-assembled gold electrode for the simultaneous determination of epinephrine and dopamine［J］. Chinese Chemical Letters, 2009,20(2):221-224.
【6】牛凌梅,康维钧,连靠奇,等.2,3-二巯基丁二酸修饰金电极伏安法同时测定肾上腺素及多巴胺［J］.理化检验-化学分册, 2010,46(11):1256-1259.
【7】马建国,彭道锋,康文,等.肾上腺素在聚氨基-β-环糊精修饰电极上的电化学行为［J］.理化检验-化学分册, 2007,43(1):1-7.
【8】牛燕,孙雪梅,孙久龙,等.毛细管电泳电化学检测多巴胺、肾上腺素和抗坏血酸［J］.青岛科技大学学报, 2008,29(3):206-209.
【9】LANE R F, HHBBARD A T. Differential double pulse voltammetry at chemically modified platinum electrodes for in vivo determination of catechol amines［J］. Anal Chem, 1976,48(9):1287-1293.
【10】马心英.循环伏安法测定去甲肾上腺素［J］.理化检验-化学分册, 2008,44(6):523-526.
【11】王玉红,孔德生,郁章玉.聚苯胺修饰玻碳电极上肾上腺素的电催化氧化［J］.菏泽学院学报, 2008,30(2):61-64.
【12】ZHANG Lei, LIN Xiang-qin. Covalent modification of glassy carbon electrode with glutamic acid for simultaneous determination of uric acid and ascorbic acid［J］. Analyst, 2001,126:367-370.
【13】VIRE J C, KAUFFMANN J M, BRAUN J, et al. Caracteris-tiques electrochimiques dun nouvel antiinflam-matoire non-steroidien:le piroxicam［J］.Analusis, 1985,13(3):134-140.
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