免疫亲和色谱-高效液相色谱法测定河水和土壤中克百威、三唑磷和绿磺隆残留量
IAC-HPLC Determination of Residual Amounts of Carbofuran, Triaophos and Chlorsulfuran in River Water and Soil
摘 要
将河水或土壤样品的丙酮提取液通过串联的分别装有克百威、三唑磷和绿磺隆免疫亲和吸着剂的3支免疫亲和色谱柱(IAC)或同时装有上述3种吸着剂的多抗体IAC,对样品中克百威、三唑磷及绿磺隆3种农药进行分离。采用串联IAC分离时,在样品溶液过柱后,将串联的IAC柱分开,并用不同配比的甲醇-水溶液分别从3支IAC柱上洗脱农药,用ELISA和HPLC分别测得农药的含量。采用多抗体IAC分离时,用甲醇-水溶液洗脱后,用上述两种方法测得洗脱液中3种农药的含量。对2种分离方法作回收和精密度试验,两方法的回收率和重复性基本一致。
高效液相色谱
克百威
三唑磷
绿磺隆
Immunoaffinity chromatography(IAC)
HPLC
Carbofuran
Triaophos
Chlorsulfuran
Abstract
Sample solution of river water or acetone extract of soil sample was passed through either a set of 3 immunoaffinity chromatographic columns (IAC) connected in series in which immunoaffinity sorbents of carbofuran, triaophos and chlorsulfuran were filled separately, or through a multi-antibody IAC in which the 3 immunoaffinity sorbents were filled together, to separate the 3 pesticides, carbofuran, triaophos and chlorsulfuran, from the sample solution. When the IAC in series was used in separation, the 3 columns were disconnected after passing-through of the sample solution, and each of the columns was eluted with mixtures of CH3OH and H2O (mixed in different ratios). Amounts of carbofuran, triaophos and chlorsulfuran were determined by ELISA and HPLC separately in the respective eluates from the columns. When the multi-antibody IAC was used in separation, the 3 pesticides in the eluate were determined simultaneously by ELISA and HPLC separately. Tests for recovery and precision for the 2 methods of separation were made, giving consistent results of recovery and repeatability between the 2 methods.
中图分类号 O657.7
所属栏目 试验与研究
基金项目 国家自然科学基金资助项目(20177019);江苏省“青蓝工程”资助顺目(2008)
收稿日期 2011/8/13
修改稿日期
网络出版日期
作者单位点击查看
备注韦林洪(1970-),女,江苏高邮人,讲师,硕士,主要研究方向为色谱技术。
引用该论文: WEI Lin-hong,LIU Shu-zhao,SHAO Xiu-jin. IAC-HPLC Determination of Residual Amounts of Carbofuran, Triaophos and Chlorsulfuran in River Water and Soil[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(8): 887～892
韦林洪,刘曙照,邵秀金. 免疫亲和色谱-高效液相色谱法测定河水和土壤中克百威、三唑磷和绿磺隆残留量[J]. 理化检验－化学分册, 2012, 48(8): 887～892
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参考文献
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【21】DAVIS G C, HEIN M B, CHAPMAR D A. Evaluation of immunosorbents for the analysis of small molecules isolation and purification of cytokinins［J］. Journal of Chromatography, 1986,366:171-189.
【2】裴道国,崔淑华,王雄,等.免疫亲合柱净化-气相色谱质谱法测定花生和大蒜中乙草胺残留量［J］.中国食品卫生杂志, 2008,20(5):418-420.
【3】刘曙照,韦林洪,徐维娜.克百威的免疫亲和色谱分析研究［J］.色谱, 2005,23(2):134-137.
【4】韦林洪,王莲,刘曙照.稻米中三唑磷残留免疫亲合色谱-高效液相色谱分析［J］.中国农业科学, 2006,39(5):941-946.
【5】冯大和,邵秀金,韦林洪,等.免疫亲和色谱-高效液相色谱法测定土壤中氯磺隆残留［J］.农业环境科学学报,2006,25(6):1663-1666.
【6】CHUANGA J C, VANEMONB J M. Development and appilication of immunoaffinity column chromatography for atrazine in complex sample medial［J］. Anal Chim Acta, 2007,583:32-39.
【7】SANCHEZ F G, HERRERA D. Development and characterisation of an immunoaffinity chromatographic column for the on-line determination of the pesticide triclopyr［J］. Talanta, 2007,71:1411-1416.
【8】KOOLE A, BOSMAN J, FRANKE J P, et al. R. A. Multiresidue analysis of β-agonists in human and calf urine using multimodal solid-phase extraction and high-performance liquid chromatography with electrochemical detection［J］. Journal of Chromatography B, 1999,726:149-156.
【9】蒋兴东,陈玉飞,涂健,等.免疫亲和色谱在动物源性食品兽药残留分析检测中的应用［J］.家禽科学, 2007(12):35-38.
【10】赵思俊,郑增忍,曲志娜,等.免疫亲和色谱-HPLC-FLD法测定动物肝脏10种喹诺酮类药物残留［J］.分析化学, 2009,37(3):335-340.
【11】DICKSON C L, MACNEIL J D, REID J, et al. Validation of screening method for residues of diethylstilbestrol, dienestrol, hexastrol, and zeranol in bovine urine using immunonity chromatography and gas chromatography/mass spectrometry［J］. J AOAC, 2003,86(5):631-638.
【12】樊磊,祁克宗,朱良强.氧氟沙星免疫亲和色谱柱的研制［J］.中国动物检疫, 2009,26(7):40-42.
【13】陈亮,陈婷,徐强.免疫亲和色谱特异性剔除中药方剂中四逆散中的柚皮苷［J］.色谱, 2006,24(3):243-246.
【14】王伟,葛宝坤,苏毅,等.免疫亲和柱净化、高效液相色谱法结合荧光检测器检测进出口芝麻中的黄曲霉毒素B1［J］.口岸卫生控制, 2007,12(1):20-21.
【15】GURBAY A, AYDIN S, GIRGIN G, et al. Assessment of aflatoxin M1 levels in milk in Ankara, Turkey［J］. Food Control, 2006,17:1-4.
【16】GINKEL L A V. Immunoaffinity chromatography, its applicability and limitations in multi-residue analysis of anabolizing and doping agents［J］. Chromatogr, 1991,564(2):363-384.
【17】韦林洪,刘曙照,邵秀金,等,绿磺隆克百威三唑磷多抗体免疫亲和色谱技术研究［J］.农业环境科学学报, 2009,28(10):2211-2216.
【18】刘曙照,冯大和,陈美娟,等.对克百威具高度特异性的免疫分析技术研究［J］.分析科学学报, 2000,16(5):373-378.
【19】刘曙照,王莲,韦林洪.三唑磷的免疫分析技术研究［J］.中国农业科学, 2005,33(12):1697-1700.
【20】刘曙照,冯大和,邵秀金.氯磺隆酶联免疫吸附分析技术研究［J］.分析科学学报, 2000,16(6):461-465.
【21】DAVIS G C, HEIN M B, CHAPMAR D A. Evaluation of immunosorbents for the analysis of small molecules isolation and purification of cytokinins［J］. Journal of Chromatography, 1986,366:171-189.
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