Rapid Photometric Determination of Long Chain Dicarboxylic Acids
摘 要
长链二元酸与硫酸铜甲醇溶液反应生成沉淀,离心去除沉淀后,于波长820 nm处测定上清液吸光度,提出了光度法间接测定长链二元酸含量的方法。长链二元酸的质量浓度在5~40 g·L-1范围内与吸光度呈线性关系,方法检出限(3s/k)为0.011 g·L-1。方法的回收率在104%~105%之间,测定值的相对标准偏差(n=5)小于0.6%。
Abstract
Long chain dicarboxylic acids reacted with Cu2+ in methanol solution of CuSO4 to form a precipitate. After centrifugation, absorbance of the supernatant was determined at 820 nm. Based on this fact, a photometric method for indirect determination of long chain dicarboxylic acids was proposed. Linear relationship between values of absorbance and mass concentration of long chain dicarboxylic acids was kept in the range of 5-40 g·L-1, with detection limit (3s/k) of 0.011 g·L-1. Values of recovery were found in the range of 104%-105%, and RSD′s (n=5) were less than 0.6%.
中图分类号 O657.32
所属栏目 工作简报
基金项目 广西化工过程创新技术研发平台建设(桂科能0992028-13);广西民族大学重点项目科研基金(200701YJ01)
收稿日期 2011/6/17
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备注林日辉(1972-),男,广西梧州人,副教授,博士,主要从事发酵工程、酶工程方面的研究。
引用该论文: LIN Ri-hui,YU Yun-ling,CHEN Li,XUAN Jin-cai. Rapid Photometric Determination of Long Chain Dicarboxylic Acids[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(8): 915~916
林日辉,余运玲,陈莉,禤金彩. 光度法快速测定长链二元酸的含量[J]. 理化检验-化学分册, 2012, 48(8): 915~916
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