反相高效液相色谱法测定中药材虎杖中白藜芦醇含量
RP-HPLC Determination of Resveratrol in TCM Polygonum Cuspidatum
摘 要
提出了反相高效液相色谱法测定虎杖中白藜芦醇含量的方法。以ODS-C18色谱柱(200 mm×4.6 mm,5 μm)为分离柱,以乙醇-水(2+3)溶液作流动相淋洗,在紫外光检测波长304 nm处进行测定。白藜芦醇的质量浓度在0.05~0.40 mg·L-1范围内与峰面积呈线性关系,方法的检出限(3S/N)为10 μg·L-1。方法用于中药材虎杖中白藜芦醇含量的测定,标准加入平均回收率为98.5%,测定值的相对标准偏差(n=5)为2.6%。
虎杖
白藜芦醇
RP-HPLC
Polygonum cuspidatum
Resveratrol
Abstract
RP-HPLC was applied to the determination of resveratrol in polygonum cuspidatum. The ODS-C18 column (200 mm×4.6 mm, 5 μm) was used as stationary phase, and a mixture of CH3CH2OH and H2O (2+3) was used as mobile phase. The content of resveratrol was determined by UV-detection at 304 nm. Linear relationship between values of peak area and mass concentration of resveratrol was kept in the range of 0.05-0.40 mg·L-1, with detection limit (3S/N) of 10 μg·L-1. The proposed method was used in the determination of resveratrol in polygonum cuspidatum, giving values of average recovery obtained by standard addition method of 98.5% and RSD (n=5) of 2.6%.
中图分类号 O652.63
所属栏目 工作简报
基金项目
收稿日期 2011/9/28
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备注白祖海(1978-),男,湖南岳阳人,主管检验师,硕士,主要研究方向为理化检测。
引用该论文: BAI Zu-hai,WEN Gang,ZHANG Yan. RP-HPLC Determination of Resveratrol in TCM Polygonum Cuspidatum[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(9): 1045～1047
白祖海,文刚,张艳. 反相高效液相色谱法测定中药材虎杖中白藜芦醇含量[J]. 理化检验－化学分册, 2012, 48(9): 1045～1047
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参考文献
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【2】KIMURA Y, KO S. Effects of stilbene components of roots of Polygonum cuspidatum on liver injury in peroxidized oil-fed rats［J］. Planta Med, 1983,48(1):51-54.
【3】GANINI S J, HIRNTHI J, EUNG S L, et al. Kinase inhibitors from polygonum cuspidatum［J］. J Nat Prod, 1993,56(10):1805-1810.
【4】张立伟,王金山,江崇球.超临界流体CO2萃取中药有效成分的抗氧化性的分光光度法研究［J］.光谱学与光谱分析, 2004,24(9):1103-1105.
【5】郭玫,余晓晖,叶建青,等.应用胶束薄层色谱分离鉴定虎杖中的成分［J］.甘肃中医学院学报, 1999,16(3):51-52.
【6】周建军,张宏杰,杨培君,等.汉中地区虎杖中自藜芦醇苷及苷元含量的测定［J］.中草药, 2002,33(5):414-416.
【7】YANG Fu-quan, ZHANG Tian-you, HO Yoichiro. Largescale separation of resveratrol, anthmglycoside A and anthraglycoside B from polygonum cuspidatum Sieb. et Zucc by high-speed counter-current chromatography［J］. J Chromatogr A, 2001,919:443-448.
【8】曹庸,于华忠,张敏,等.HPLC法测定虎杖白藜芦醇的含量及其稳定性研究［J］.林产化学与工业, 2004,24(2):61-64.
【9】杨红美,陈波,曾建国,等.HPLC同时测定虎杖及其提取物中4种有效成分的含量［J］.中国中药杂志, 2006,31(3):202-205.
【10】肖凯,宣利江,徐亚明,等.虎杖的化学成分研究［J］.中国药学杂志, 2003,38(1):12-14.
【2】KIMURA Y, KO S. Effects of stilbene components of roots of Polygonum cuspidatum on liver injury in peroxidized oil-fed rats［J］. Planta Med, 1983,48(1):51-54.
【3】GANINI S J, HIRNTHI J, EUNG S L, et al. Kinase inhibitors from polygonum cuspidatum［J］. J Nat Prod, 1993,56(10):1805-1810.
【4】张立伟,王金山,江崇球.超临界流体CO2萃取中药有效成分的抗氧化性的分光光度法研究［J］.光谱学与光谱分析, 2004,24(9):1103-1105.
【5】郭玫,余晓晖,叶建青,等.应用胶束薄层色谱分离鉴定虎杖中的成分［J］.甘肃中医学院学报, 1999,16(3):51-52.
【6】周建军,张宏杰,杨培君,等.汉中地区虎杖中自藜芦醇苷及苷元含量的测定［J］.中草药, 2002,33(5):414-416.
【7】YANG Fu-quan, ZHANG Tian-you, HO Yoichiro. Largescale separation of resveratrol, anthmglycoside A and anthraglycoside B from polygonum cuspidatum Sieb. et Zucc by high-speed counter-current chromatography［J］. J Chromatogr A, 2001,919:443-448.
【8】曹庸,于华忠,张敏,等.HPLC法测定虎杖白藜芦醇的含量及其稳定性研究［J］.林产化学与工业, 2004,24(2):61-64.
【9】杨红美,陈波,曾建国,等.HPLC同时测定虎杖及其提取物中4种有效成分的含量［J］.中国中药杂志, 2006,31(3):202-205.
【10】肖凯,宣利江,徐亚明,等.虎杖的化学成分研究［J］.中国药学杂志, 2003,38(1):12-14.
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