高效液相色谱-手性流动相添加法拆分盐酸丙胺卡因对映体
Resolution of Enantiomers of Propitocaine Hydrochloride by HPLC Using Mobile Phase Added with Chiral Additive
摘 要
以羧甲基-β-环糊精(CM-β-CD)为手性流动相添加剂,提出了拆分盐酸丙胺卡因对映体的高效液相色谱法。选用C18色谱柱,流动相为含0.4 g·L-1的CM-β-CD的甲醇-乙腈-水(60+15+25)溶液,紫外检测波长为202 nm,进样量为10 μL,流量为0.4 mL·min-1。结果表明:盐酸丙胺卡因对映体得到良好的分离,分离度为1.41。
高效液相色谱法
对映体拆分
羧甲基-β-环糊精
盐酸丙胺卡因
Chiral additive for mobile phase
HPLC
Enantiomer resolution
CM-β-CD
Propitocaine hydrochloride
Abstract
Carboxymethyl-β-cyclodextrin (CM-β-CD) was used as chiral additive for the mobile phase in the resolution of enantiomers of propitocaine hydrochloride by HPLC. The C18 column was used as stationary phase, and a mixture of methanol, acetonitrile and water (mixed in the ratio of 60+15+25) containing 0.4 g·L-1 CM-β-CD was used as mobile phase, at a flow-rate of 0.4 mL·min-1. UV-detection at the wavelength of 202 nm was adopted for the determination, and volume of sample introduced was 10 μL. It was shown that enantiomers of propitocaine hydrochloride were successfully separated by the proposed method and values of separation factor achieved to 1.41.
中图分类号 O657.32
所属栏目 工作简报
基金项目 黑龙江省自然科学基金项目(B200911)
收稿日期 2011/9/13
修改稿日期
网络出版日期
作者单位点击查看
联系人作者苏立强(slq202@163.com)
备注苏立强(1962-),女,山东人,教授,硕士生导师,主要从事色谱理论与应用研究。
引用该论文: SU Li-qiang,SUN Yu-jie,ZHAI Ming-hui. Resolution of Enantiomers of Propitocaine Hydrochloride by HPLC Using Mobile Phase Added with Chiral Additive[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(11): 1283～1285
苏立强,孙玉杰,翟明翚. 高效液相色谱-手性流动相添加法拆分盐酸丙胺卡因对映体[J]. 理化检验－化学分册, 2012, 48(11): 1283～1285
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参考文献
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【3】PARKER J F, VATS A, BAUER G. EMLA toxicity after application for allergy skin testing［J］. Pediatrics, 2004,113(2):410-411.
【4】孙淑娟,谷大建,杨久丽,等.高效液相色谱法测定复方利多卡因乳膏中丙胺卡因与利多卡因的含量［J］.中国医院药学杂志, 2002,22(11):675-677.
【5】杨莹莹,张文胜,叶利明.气相色谱-质谱法同时测定透皮接收液中的丙胺卡因和利多卡因［J］.色谱, 2009,27(1):74-77.
【6】AMINI A, PAULSEN S U. Enantioseparation of local anaesthetic drugs by capillary zone electrophoresis with cyclodextrins as chiral selectors using a partial filling technique［J］. Electrophoresis, 1997,18(6):1019-1025.
【7】朱军峰,安文汀,张国梅,等.羧甲基-环糊精的干法制备［J］.应用化学, 2008,25(6):681-684.
【8】雷雯,张凌怡,万莉,等.伊瑞霉素键合手性毛细管整体柱的制备与对映体分离［J］.色谱, 2010,28(10):977-983.
【2】DOKO Y, IRANAMI H, FUJII K, et al. Severe methemoglobinemia after dental anesthesia: a warning about propitocaine-induced methemoglobinemia in neonates［J］. Journal of Anesthesia, 2010,24(6):935-937.
【3】PARKER J F, VATS A, BAUER G. EMLA toxicity after application for allergy skin testing［J］. Pediatrics, 2004,113(2):410-411.
【4】孙淑娟,谷大建,杨久丽,等.高效液相色谱法测定复方利多卡因乳膏中丙胺卡因与利多卡因的含量［J］.中国医院药学杂志, 2002,22(11):675-677.
【5】杨莹莹,张文胜,叶利明.气相色谱-质谱法同时测定透皮接收液中的丙胺卡因和利多卡因［J］.色谱, 2009,27(1):74-77.
【6】AMINI A, PAULSEN S U. Enantioseparation of local anaesthetic drugs by capillary zone electrophoresis with cyclodextrins as chiral selectors using a partial filling technique［J］. Electrophoresis, 1997,18(6):1019-1025.
【7】朱军峰,安文汀,张国梅,等.羧甲基-环糊精的干法制备［J］.应用化学, 2008,25(6):681-684.
【8】雷雯,张凌怡,万莉,等.伊瑞霉素键合手性毛细管整体柱的制备与对映体分离［J］.色谱, 2010,28(10):977-983.
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