提出了高效液相色谱-串联质谱法测定人尿液中鸦片类、苯丙胺类和可卡因类等28种毒品含量的方法。试样用乙酸乙酯提取,离心分离,提取液于40 ℃氮气吹干后,用甲醇-水(30+70)溶液1 mL溶解定容,用高效液相色谱-串联质谱检测。28种毒品的质量分数均在2.0~200 μg·kg-1范围内呈线性关系,方法的检出限(3S/N)在1.0~2.0 μg·kg-1之间。在空白尿液样品中加入5,10,50 μg·kg-1混合标准溶液,测得28种毒品的回收率在61.0%~117%之间,相对标准偏差(n=6)在3.0%~14%之间。

A method of HPLC-MS/MS for the determination of 28 abused drugs, such as opioids, amphetamines and cocaines, in human urine was proposed. The sample was extracted with ethyl acetate, after centrifugation, the supernatant was taken and evaporated to dryness by N2-blowing at 40 ℃. The residue was taken up with 1.0 mL of a mixture of methanol and water (30+70) and analyzed by HPLC-MS/MS. Linear relationships between values of peak area and mass fraction of 28 abused drugs were kept in the same range of 2.0-200 μg·kg-1, with detection limits (3S/N) in the range of 1.0-2.0 μg·kg-1. Recovery and precision of the method were tested by addition of mixed abused drugs standards at 3 concentration levels of 5,10,50 μg·kg-1, giving values of recovery in the range of 61.0%-117% with RSD′s (n=6) in the range of 3.0%-14%.