加压毛细管电色谱法分离盐酸奥昔布宁对映体
Resolution of Enantiomers of Oxybutynin Chloride by Pressurized Capillary Electrochromatography
摘 要
采用加压毛细管电色谱法,以羟丙基-β-环糊精作为手性选择剂,对盐酸奥昔布宁对映体进行手性分离。在优化的试验条件下,16 g·L-1羟丙基-β-环糊精为手性选择剂,pH 2.8的乙腈-5 mmol·L-1磷酸盐缓冲溶液以体积比60比40混合溶液为流动相,运行电压18 kV,毛细管柱温度18 ℃,盐酸奥昔布宁对映体在14 min内成功分离。
盐酸奥昔布宁
对映体拆分
羟丙基-β-环糊精
Pressurized capillary electrochromatography
Oxybutynin chloride
Resolution of enantiomers
Hydroxypropyl-β-cyclodextrin
Abstract
A method of pressurized capillary electrochromatography was proposed for the resolution of enantiomers of oxybutynin chloride using hydroxypropyl-β-cyclodextrin (16 g·L-1) as selective chiral agent. A mixed solution of acetonitrile and 5 mmol·L-1 phosphate buffer solution (60+40, by vol.) of pH 2.8 was used as mobile phase. Separation voltage of 18 kV and temperature of capillary column of 18 ℃ were chosen. Under the optimized conditions, the enantiomers of oxybutynin chloride were successfully separated within 14 min.
中图分类号 O657.7
所属栏目 工作简报
基金项目
收稿日期 2011/7/2
修改稿日期
网络出版日期
作者单位点击查看
联系人作者高玉玲(iam_0@163.com)
备注高玉玲(1983-),女,黑龙江人,研究实习员,硕士,主要从事色谱方向的研究。
引用该论文: GAO Yu-ling. Resolution of Enantiomers of Oxybutynin Chloride by Pressurized Capillary Electrochromatography[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(11): 1324～1325
高玉玲. 加压毛细管电色谱法分离盐酸奥昔布宁对映体[J]. 理化检验－化学分册, 2012, 48(11): 1324～1325
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参考文献
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【2】KAPNISSI C P, VALLE B C, WARNER I M. Chiral separations using polymeric surfactantsand polyelectrolyte multilayers in open-tubular capillary electrochromatography［J］. Anal Chem, 2003,75:6097-6104.
【3】吴漪,张晓晖,魏娟,等.毛细管电色谱和加压毛细管电色谱的进展与应用［J］.色谱, 2009,27(5):609-611.
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【5】HAYASHI A, SAITO M. Treatment with modified intravesical oxybutynin chloride for neurogenic bladder in children［J］. Journal of Pediatric Urology, 2007(3):438-441.
【6】KIM H, HAN S B, PHARM J. Sensitive determination of oxybutynin and desethyloxybutynin in dog plasma by LC-ESI/MS/MS［J］. Journal of Pharmaceutical and Biomedical Analysis, 2003,31(2):341-344.
【7】WALKER T A. The chiral separation of oxybutynin enantiomers using an ovomucoid column［J］. Journal of Liquid Chromatography & Related Technologies, 2000,23(6):841-845.
【8】郭娜,高新星,徐国防,等.高效液相色谱手性流动相添加法拆分奥昔布宁对映体［J］.色谱, 2008,26(2):259-261.
【9】高立娣,孙鹏,李秋芳,等.高效毛细管电泳法手性拆分奥昔布宁对映体［J］.应用化学, 2009,26(8):1002-1004.
【2】KAPNISSI C P, VALLE B C, WARNER I M. Chiral separations using polymeric surfactantsand polyelectrolyte multilayers in open-tubular capillary electrochromatography［J］. Anal Chem, 2003,75:6097-6104.
【3】吴漪,张晓晖,魏娟,等.毛细管电色谱和加压毛细管电色谱的进展与应用［J］.色谱, 2009,27(5):609-611.
【4】温清,贺坤,郭瑞臣.盐酸奥昔布宁临床应用进展［J］.中国药事, 2004,18(7):445-447.
【5】HAYASHI A, SAITO M. Treatment with modified intravesical oxybutynin chloride for neurogenic bladder in children［J］. Journal of Pediatric Urology, 2007(3):438-441.
【6】KIM H, HAN S B, PHARM J. Sensitive determination of oxybutynin and desethyloxybutynin in dog plasma by LC-ESI/MS/MS［J］. Journal of Pharmaceutical and Biomedical Analysis, 2003,31(2):341-344.
【7】WALKER T A. The chiral separation of oxybutynin enantiomers using an ovomucoid column［J］. Journal of Liquid Chromatography & Related Technologies, 2000,23(6):841-845.
【8】郭娜,高新星,徐国防,等.高效液相色谱手性流动相添加法拆分奥昔布宁对映体［J］.色谱, 2008,26(2):259-261.
【9】高立娣,孙鹏,李秋芳,等.高效毛细管电泳法手性拆分奥昔布宁对映体［J］.应用化学, 2009,26(8):1002-1004.
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