离子色谱法测定减肥食品中左旋肉碱
IC Determination of L-Carnitine in Slimming Diet
摘 要
提出了离子色谱法测定减肥食品中左旋肉碱含量的方法。样品用超纯水于30 ℃超声提取30 min,经离心分离,取上清液通过OnGuard II RP柱净化。流出液以IonPac CS17柱为离子交换柱,5 mmol·L-1甲基磺酸溶液为淋洗液,采用非抑制电导检测器进行检测。左旋肉碱的质量浓度在10~1 000 mg·L-1范围内与其峰面积呈线性关系,检出限(3S/N)为1 mg·g-1。以两种减肥产品为基体,进行加标回收试验,所得回收率在95.4%~101%之间,测定值的相对标准偏差(n=5)在0.9%~6.6%之间。
左旋肉碱
减肥产品
IC
L-carnitine
Slimming diet
Abstract
A method of IC for determination of L-carnitine in slimming diet was proposed. The sample was extracted with ultrapure water for 30 min at 30 ℃, after centrifugation, the supernatant was purified by passing through OnGuard II RP column. IonPac CS17 column was used for separation with 5 mmol·L-1 methylsulfonic acid solution as eluant. Non-inhibitory conductivity detection was adopted in the determination. Linear relationship between values of peak area and mass concentration of L-carnitine was obtained in the range of 10-1 000 mg·L-1, with detection limit (3S/N) of 1 mg·g-1. Using 2 samples of slimming diet as matrixes, test for recovery was made by standard addition method, giving values of recovery and RSD′s (n=5) in ranges of 95.4%-101% and 0.9%-6.6% respectively.
中图分类号 O652.63
所属栏目 试验与研究
基金项目
收稿日期 2011/12/7
修改稿日期
网络出版日期
作者单位点击查看
备注陈军(1977-),男,湖北荆门人,应用工程师,主要从事液相、离子色谱方法的开发与应用。
引用该论文: CHEN Jun,ZHONG Xin-lin,YU Yan-hai. IC Determination of L-Carnitine in Slimming Diet[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(1): 6～10
陈军,钟新林,余彦海. 离子色谱法测定减肥食品中左旋肉碱[J]. 理化检验－化学分册, 2013, 49(1): 6～10
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】周友亚,王雪.左旋肉碱的生理功能及应用进展［J］.河北师范大学学报:自然科学版, 2001,25(1):99-101．
【2】WOOLLARD D C, INDYK H E, WOOLLARD G A. Enzymatic determination of carnitine in milk and infant formula［J］. Food Chem, 1997,59(3):325-332．
【3】SELINE K G, JOHEIN H. The determination of l-carnitine in several food samples［J］. Food Chemistry, 2007,105:793-804．
【4】JOS A P, FERNANDO A, LUIS A E, et al. Determination of free and total carnitine in plasma by an enzymatic reaction and spectrophotometric quantitation spectrophotometric determination of carnitine［J］. Clinical Biochemistry, 2006,39:1022-1027．
【5】WORAPAN P, RJANE M, STEPHAN K, et al. Capillary electrophoresis with contactless conductivity detection for the determination of carnitine and acylcarnitines in clinical samples［J］. Journal of Chromatography B, 2011,879:921-926．
【6】LAURA S H, MARA C P, CARMEN G R, et al. Determination of L- and D-carnitine in dietary food supplements using capillary electrophoresis-tandem mass spectrometry［J］. Food Chemistry, 2010,120:921-928．
【7】DAVID W J. An acid hydrolysis method for quantification of plasma free and total carnitine by flow injection tandem mass spectrometry［J］. Clinical Biochemistry, 2010,43:1362-1367．
【8】ALEXANDER A R, STEFAN R I, STADEN J F, et al. Determination of L-carnitine using enantioselective, potentiometric membrane electrodes based on macrocyclic antibiotics［J］. Talanta, 2004,63:515-519．
【9】邹晓莉,周艳阳,乔蓉,等.高效阴离子色谱法快速分析保健食品中的左旋肉碱［J］.卫生研究, 2011,40(3):358-364．
【10】HE G X, DAHL T. Improved high performance liquid chromatographic method for analysis of L-carnitine in pharmaceutical formulations［J］. J Pharm Biomed Anal, 2000,23:315-321．
【11】KAKOU A, MEGOULAS N C, KOUPPARIS M A, et al. Determination of L-carnitine in food supplement formulations using ion-pair chromatography with indirect conductimetric detection［J］. J Chromatogr A, 2005,1069:209-215．
【12】祝伟霞,杨冀州,刘亚风,等.保健品种左旋肉碱的反相高效液相色谱法测定［J］.分析测试报, 2008,27(10):1124-1127．
【13】徐娟娟,李杰梅,梁淑明,等.手性高效液相色谱法测定左旋肉碱中光学异构体的含量［J］.现代食品科技, 2010,26(3):311-313．
【14】徐连明,王振中,毕宇安,等.HPLC法测定减肥胶囊中左旋肉碱的含量［J］.海峡药学, 2009,21(11):47-48．
【15】甘宾宾,黎少豪.HPLC法测定保健食品中左旋肉碱含量的研究［J］.中国卫生检验杂志, 2010,20(7):1688-1689．
【16】黄芳,黄晓兰,吴慧勤,等.液相色谱-质谱法快速测定婴幼儿配方食品中L-肉碱的亲水相互作用［J］.分析试验室, 2011,30(10):111-114．
【17】LI Ke, LI Wei, HUANG Yu-feng. Determination of free L-carnitine in human seminal plasma by high performance liquid chromatography with pre-column ultraviolet derivatization and its clinical application in male infertility［J］. Clinica Chimica, 2007,3378:159-163．
【18】胡晓娟,张锦,赵阗.反相高效液相色谱法测定保健品中左旋肉碱的含量［J］.光谱实验室, 2011,28(2):743-746．
【2】WOOLLARD D C, INDYK H E, WOOLLARD G A. Enzymatic determination of carnitine in milk and infant formula［J］. Food Chem, 1997,59(3):325-332．
【3】SELINE K G, JOHEIN H. The determination of l-carnitine in several food samples［J］. Food Chemistry, 2007,105:793-804．
【4】JOS A P, FERNANDO A, LUIS A E, et al. Determination of free and total carnitine in plasma by an enzymatic reaction and spectrophotometric quantitation spectrophotometric determination of carnitine［J］. Clinical Biochemistry, 2006,39:1022-1027．
【5】WORAPAN P, RJANE M, STEPHAN K, et al. Capillary electrophoresis with contactless conductivity detection for the determination of carnitine and acylcarnitines in clinical samples［J］. Journal of Chromatography B, 2011,879:921-926．
【6】LAURA S H, MARA C P, CARMEN G R, et al. Determination of L- and D-carnitine in dietary food supplements using capillary electrophoresis-tandem mass spectrometry［J］. Food Chemistry, 2010,120:921-928．
【7】DAVID W J. An acid hydrolysis method for quantification of plasma free and total carnitine by flow injection tandem mass spectrometry［J］. Clinical Biochemistry, 2010,43:1362-1367．
【8】ALEXANDER A R, STEFAN R I, STADEN J F, et al. Determination of L-carnitine using enantioselective, potentiometric membrane electrodes based on macrocyclic antibiotics［J］. Talanta, 2004,63:515-519．
【9】邹晓莉,周艳阳,乔蓉,等.高效阴离子色谱法快速分析保健食品中的左旋肉碱［J］.卫生研究, 2011,40(3):358-364．
【10】HE G X, DAHL T. Improved high performance liquid chromatographic method for analysis of L-carnitine in pharmaceutical formulations［J］. J Pharm Biomed Anal, 2000,23:315-321．
【11】KAKOU A, MEGOULAS N C, KOUPPARIS M A, et al. Determination of L-carnitine in food supplement formulations using ion-pair chromatography with indirect conductimetric detection［J］. J Chromatogr A, 2005,1069:209-215．
【12】祝伟霞,杨冀州,刘亚风,等.保健品种左旋肉碱的反相高效液相色谱法测定［J］.分析测试报, 2008,27(10):1124-1127．
【13】徐娟娟,李杰梅,梁淑明,等.手性高效液相色谱法测定左旋肉碱中光学异构体的含量［J］.现代食品科技, 2010,26(3):311-313．
【14】徐连明,王振中,毕宇安,等.HPLC法测定减肥胶囊中左旋肉碱的含量［J］.海峡药学, 2009,21(11):47-48．
【15】甘宾宾,黎少豪.HPLC法测定保健食品中左旋肉碱含量的研究［J］.中国卫生检验杂志, 2010,20(7):1688-1689．
【16】黄芳,黄晓兰,吴慧勤,等.液相色谱-质谱法快速测定婴幼儿配方食品中L-肉碱的亲水相互作用［J］.分析试验室, 2011,30(10):111-114．
【17】LI Ke, LI Wei, HUANG Yu-feng. Determination of free L-carnitine in human seminal plasma by high performance liquid chromatography with pre-column ultraviolet derivatization and its clinical application in male infertility［J］. Clinica Chimica, 2007,3378:159-163．
【18】胡晓娟,张锦,赵阗.反相高效液相色谱法测定保健品中左旋肉碱的含量［J］.光谱实验室, 2011,28(2):743-746．
相关信息
