Preparation of Modified GCE by Composite Film of CTS/DNA/Hb/MWCNTs and Study on Its Electrochemical Behavior
摘 要
将多壁碳纳米管(MWCNTs)分散在壳聚糖(CTS)中并滴涂在玻碳电极表面,烘干后依次滴加血红蛋白(Hb)、DNA及CTS溶液,制成了壳聚糖/DNA/血红蛋白/多壁碳纳米管复合膜修饰的GCE(简示为CTS/DNA/Hb/MWCNTs/GCE)。采用方波伏安法及循环伏安法研究了膜内DNA的电化学行为。结果表明:在pH 5.8的磷酸盐缓冲溶液中,复合膜内的DNA在电极上于0.46 V(vs.SCE)处有一个明显的氧化峰,DNA氧化峰电流与其质量在1.0~5.0 μg范围内呈线性关系,检出限(3S/N)为0.5 μg。
Abstract
Multi-walled carbon nanotubes (MWCNTs) were dispersed in chitosan (CTS) and dropped on the surface of glassy carbon electrode (GCE), after drying solutions of hemoglobin (Hb), DNA and CTS were successively dropped on the above electrode, to form the composite film modified GCE or named abbreviately as CTS/DNA/Hb/ MWCNTs/GCE. The electrochemical property of the modified electrode was studied by square wave voltammetry and cyclic voltammetry. It was found that DNA in film showed an obvious oxidative peak at the potential of 0.46 V (vs.SCE) in phosphate buffer solution of pH 5.8. Linear relationship between values of oxidative peak current and mass of DNA in the composite film was obtained in the range of 1.0-5.0 μg. Value of detection limit (3S/N) was found 0.5 μg.
中图分类号 O657.1
所属栏目 试验与研究
基金项目 山东省自然科学基金资助项目(ZR2009BM003);国家自然科学基金资助项目(21105023)
收稿日期 2012/4/27
修改稿日期
网络出版日期
作者单位点击查看
联系人作者焦翠玲(yuzywxl@163.com)
备注焦翠玲(1986-),女,山东东营人,硕士研究生,研究方向为电分析化学。
引用该论文: JIAO Cui-ling,WANG Xue-liang,TONG Yuan-hui,YU Zhang-yu. Preparation of Modified GCE by Composite Film of CTS/DNA/Hb/MWCNTs and Study on Its Electrochemical Behavior[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(1): 15~18
焦翠玲,王学亮,同元辉,郁章玉. 壳聚糖/DNA/血红蛋白/多壁碳纳米管复合膜修饰玻碳电极的制备及其电化学行为的研究[J]. 理化检验-化学分册, 2013, 49(1): 15~18
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】刘曙照,孔红山.液相色谱法测定DNA碱基比例及相关条件的优化[J].分析测试学报, 1995,14(3):15-19.
【2】HU Jun-jie, ZHANG Wen-bing, MA Hui-min, et al. Simultaneous determination of 8-hydroxy-2′-deoxyguanosine and 5-methyl-2′-deoxycytidine in DNA sample by high performance liquid chromatography/positive electrospray ionization tandem mass spectrometry[J]. J Chromatogr B, 2010,878(28):2765-2769.
【3】PAWEL J, MIRAL D. Identification and quantification of (5′R)- and (5′S)-8,5′-cyclo-2′-deoxyadenosines in human urine as putative biomarkers of oxidatively induced damage to DNA[J]. Biochem Biophys Res Commun, 2010,397(1):48-52.
【4】赵洪桃,张志洁,鞠火晃先.基于长程电子传递的DNA直接电化学检测[J].科学通报, 2005,50(14):1539-1541.
【5】YANG Jie, WANG Xue-liang, SHI Hua-qiang. An electrochemical DNA biosensor for highly sensitive detection of phosphinothricin acetyltransferase gene sequence based on polyaniline-(mesoporous nanozirconia)/poly-tyrosine film[J]. Sensors and Actuators B, 2012,162:178-183.
【6】王学亮,杨婕.DNA电化学生物传感器在转基因植物特定序列基因检测中的应用[J].理化检验-化学分册, 2011,47(2):133-138.
【7】QIN Pin-zhu, NIU Cheng-gang, ZENG Guang-ming, et al. Time-resolved fluorescence based DNA detection using novel europium ternary complex doped silica nanoparticles[J]. Talanta, 2009,80(2):991-995.
【8】YANG Yu-bin, JI Shao-min, ZHOU Fu-ke, et al. Synthesis of novel bispyrene diamines and their application as rationmetric fluorescent probes for detection of DNA[J]. Biosens Bioelectron, 2009,24(12):3442-3447.
【9】龙云飞,陈小明,伍群丽,等.碱蓝6B共振光散射法检测DNA[J].光谱学与光谱分析, 2003,23(3):458-460.
【10】YU Jing-hua, LI Bo, DAI Ping, et al. Study on the interaction among molecules and the determination of DNA by light scattering with a new type rhodanine derivative[J]. Spectrochim Acta, Part A, 2009,74(4):875-880.
【11】QI Hai-bo, BI Ning, CHEN Yan-hua, et al. Determination of DNA based on localized surface plasmon resonance light scattering using unmodified gold bipyramids[J]. Spectrochim Acta, Part A, 2011,81(1):769-773.
【12】曹玮,苏明明,张书圣.芯片毛细管电泳快速定量检测DNA[J].分析化学, 2009,37(A03):389-389.
【13】SARAH M, MARIAN M, RUTH B, et al. Quantitative, small-scale, fluorophore-assisted carbohydrate electrophoresis implemented on a capillary electrophoresis-based DNA sequence analyzer[J]. Anal Biochem, 2011,413(2):104-113.
【14】ZHANG Xu-zhi, LIU Shu-feng, JIAO Kui, et al. Rapid and cost-effective detection of Sequences-specific DNA by monitoring the electrochemical response of 20-deoxyguanosine 50-triphosphate in a PCR sample[J]. Analyst, 2008,133:1729-1735.
【15】DAI Zhi-hui, LIU Song-qin, JU Huang-xian, et al. Direct electron transfer reactivity and enzymatic activity of hemoglobin in a hexagonal mesoporous silica matrix[J]. Biosens Bioelectron, 2004,19:861-867.
【16】张旭志,赵常志,焦奎,等.基于碳纳米管修饰电极/鸟嘌呤电化学体系的固体电位型pH传感器[J].分析化学, 2008,36(2):197-200.
【2】HU Jun-jie, ZHANG Wen-bing, MA Hui-min, et al. Simultaneous determination of 8-hydroxy-2′-deoxyguanosine and 5-methyl-2′-deoxycytidine in DNA sample by high performance liquid chromatography/positive electrospray ionization tandem mass spectrometry[J]. J Chromatogr B, 2010,878(28):2765-2769.
【3】PAWEL J, MIRAL D. Identification and quantification of (5′R)- and (5′S)-8,5′-cyclo-2′-deoxyadenosines in human urine as putative biomarkers of oxidatively induced damage to DNA[J]. Biochem Biophys Res Commun, 2010,397(1):48-52.
【4】赵洪桃,张志洁,鞠火晃先.基于长程电子传递的DNA直接电化学检测[J].科学通报, 2005,50(14):1539-1541.
【5】YANG Jie, WANG Xue-liang, SHI Hua-qiang. An electrochemical DNA biosensor for highly sensitive detection of phosphinothricin acetyltransferase gene sequence based on polyaniline-(mesoporous nanozirconia)/poly-tyrosine film[J]. Sensors and Actuators B, 2012,162:178-183.
【6】王学亮,杨婕.DNA电化学生物传感器在转基因植物特定序列基因检测中的应用[J].理化检验-化学分册, 2011,47(2):133-138.
【7】QIN Pin-zhu, NIU Cheng-gang, ZENG Guang-ming, et al. Time-resolved fluorescence based DNA detection using novel europium ternary complex doped silica nanoparticles[J]. Talanta, 2009,80(2):991-995.
【8】YANG Yu-bin, JI Shao-min, ZHOU Fu-ke, et al. Synthesis of novel bispyrene diamines and their application as rationmetric fluorescent probes for detection of DNA[J]. Biosens Bioelectron, 2009,24(12):3442-3447.
【9】龙云飞,陈小明,伍群丽,等.碱蓝6B共振光散射法检测DNA[J].光谱学与光谱分析, 2003,23(3):458-460.
【10】YU Jing-hua, LI Bo, DAI Ping, et al. Study on the interaction among molecules and the determination of DNA by light scattering with a new type rhodanine derivative[J]. Spectrochim Acta, Part A, 2009,74(4):875-880.
【11】QI Hai-bo, BI Ning, CHEN Yan-hua, et al. Determination of DNA based on localized surface plasmon resonance light scattering using unmodified gold bipyramids[J]. Spectrochim Acta, Part A, 2011,81(1):769-773.
【12】曹玮,苏明明,张书圣.芯片毛细管电泳快速定量检测DNA[J].分析化学, 2009,37(A03):389-389.
【13】SARAH M, MARIAN M, RUTH B, et al. Quantitative, small-scale, fluorophore-assisted carbohydrate electrophoresis implemented on a capillary electrophoresis-based DNA sequence analyzer[J]. Anal Biochem, 2011,413(2):104-113.
【14】ZHANG Xu-zhi, LIU Shu-feng, JIAO Kui, et al. Rapid and cost-effective detection of Sequences-specific DNA by monitoring the electrochemical response of 20-deoxyguanosine 50-triphosphate in a PCR sample[J]. Analyst, 2008,133:1729-1735.
【15】DAI Zhi-hui, LIU Song-qin, JU Huang-xian, et al. Direct electron transfer reactivity and enzymatic activity of hemoglobin in a hexagonal mesoporous silica matrix[J]. Biosens Bioelectron, 2004,19:861-867.
【16】张旭志,赵常志,焦奎,等.基于碳纳米管修饰电极/鸟嘌呤电化学体系的固体电位型pH传感器[J].分析化学, 2008,36(2):197-200.
相关信息