溶液捕集-高效液相色谱法测定卷烟主流烟气中主要羰基化合物
HPLC Determination of Major Carbonyl Compounds in Mainstream of Cigarette Smoke with Solvent Trapping
摘 要
卷烟主流烟气中的羰基化合物用含2,4-二硝基苯肼的乙腈-磷酸溶液为吸收液进行捕集,并在此溶液中使羰基化合物衍生化,所得衍生化产物供高效液相色谱分析。采用Merck Lichrosphere RP C18色谱柱进行分离,并用不同配比混合的水-乙腈-四氢呋喃-异丙醇(59+30+10+1)、水-乙腈-四氢呋喃-异丙醇(33+65+1+1)和乙腈的混合液作为流动相进行梯度淋洗,用紫外检测器于波长365 nm处测定。方法的检出限(3S/N)在0.04~0.12 μg·g-1之间。方法用于卷烟主流烟气的分析,回收率在75.0%~96.0%之间,测定值的相对标准偏差(n=5)在2.0%~9.3%之间。
羰基化合物
卷烟烟气
2,4-二硝基苯肼
HPLC
Carbonyl compounds
Cigarette smoke
2,4-Dinitrophenylhydrazine
Abstract
Carbonyl compounds in mainstream of cigarette smoke were trapped in a mixture solution of acetonitrile and H3PO4 (containing 2,4-dinitrophenylhydrazine), and derivatized simultaneously therein. The derivatized products obtained were taken for HPLC analysis. Merck Lichrosphere RP C18 column was used as separation column, and mixtures of component A, B and C mixed in various ratios were used in the gradient elution. Component A and B are mixtures of water, acetonitrile, tetrahydrofuran and iso-propand with 2 different ratios of (59+30+10+1) and (33+65+1+1) respectively, and component C is acetonitrile. UV-detection with the wavelength of 365 nm was adopted in the determination. Values of detection limit (3S/N) of the method were found in the range of 0.04-0.12 μg·g-1. The proposed method was applied to the analysis of mainstream of cigarette smoke, giving values of recovery in the range of 75.0%-96.0% and RSD′s (n=5) in the range of 2.0%-9.3%.
中图分类号 O652.63
所属栏目 工作简报
基金项目
收稿日期 2011/10/25
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备注张洪非(1983-),男,河南平顶山人,硕士,工程师,主要研究方向为烟草化学分析。
引用该论文: ZHANG Hong-fei,HOU Hong-wei,PANG Yong-qiang,JIANG Xing-yi,TANG Gang-ling. HPLC Determination of Major Carbonyl Compounds in Mainstream of Cigarette Smoke with Solvent Trapping[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(1): 19～22
张洪非,侯宏卫,庞永强,姜兴益,唐纲岭. 溶液捕集-高效液相色谱法测定卷烟主流烟气中主要羰基化合物[J]. 理化检验－化学分册, 2013, 49(1): 19～22
被引情况:
【1】肖卫强,黄健,袁凯龙,胡安福,吴希美,刘金莉,夏倩,戴路,周国俊, "高效液相色谱-串联质谱法测定尿液中丙烯醛的代谢产物",理化检验－化学分册 52, 11-14(2016)
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参考文献
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【2】王彦亭,谢剑平,张虹,等.降低卷烟烟气中有害成分的技术研究及应用［J］.中国烟草学报, 2003,9(3):1-7．
【3】MIYAKE, TAKASHI, YASHUHARA, et al. Gas chromatographic analysis of acrolein as 1-methyl-2- pyrazoline in cigarette smoke［J］. Kankyo Kagaku, 1995,5(3):569-573．
【4】ZHOU Dong-ji, MOLDOVEANU S. Gas chromatography-mass spectrometry of carbonyl compounds in cigarette mainstream smoke after derivation with 2,4-dinitrophenyl hydrazine［J］. Journal of Chromatography, 2004,1027:25-35．
【5】谢复炜,吴鸣,王昇,等.卷烟主流烟气中主要羰基化合物的改进分析方法［J］.中国烟草学报, 2006,12(5):15-24．
【6】徐济仓,侯英,杨式华,等.高效液相色谱法测定卷烟主流烟气中的低分子醛酮类化合物［J］.环境化学, 2006,25(6):678-682．
【7】YC/T 254-2008卷烟主流烟气中主要羰基化合物的测定［S］．
【8】杨卫花,陈章玉,徐若飞,等.高效液相色谱法测定卷烟粘合剂中甲醛、乙醛的含量［J］.理化检验-化学分册, 2010,46(2):158-160．
【2】王彦亭,谢剑平,张虹,等.降低卷烟烟气中有害成分的技术研究及应用［J］.中国烟草学报, 2003,9(3):1-7．
【3】MIYAKE, TAKASHI, YASHUHARA, et al. Gas chromatographic analysis of acrolein as 1-methyl-2- pyrazoline in cigarette smoke［J］. Kankyo Kagaku, 1995,5(3):569-573．
【4】ZHOU Dong-ji, MOLDOVEANU S. Gas chromatography-mass spectrometry of carbonyl compounds in cigarette mainstream smoke after derivation with 2,4-dinitrophenyl hydrazine［J］. Journal of Chromatography, 2004,1027:25-35．
【5】谢复炜,吴鸣,王昇,等.卷烟主流烟气中主要羰基化合物的改进分析方法［J］.中国烟草学报, 2006,12(5):15-24．
【6】徐济仓,侯英,杨式华,等.高效液相色谱法测定卷烟主流烟气中的低分子醛酮类化合物［J］.环境化学, 2006,25(6):678-682．
【7】YC/T 254-2008卷烟主流烟气中主要羰基化合物的测定［S］．
【8】杨卫花,陈章玉,徐若飞,等.高效液相色谱法测定卷烟粘合剂中甲醛、乙醛的含量［J］.理化检验-化学分册, 2010,46(2):158-160．
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