Rapid Determination of Residual Amount of Antibiotic in Milk by Piezoelectric Sensor
摘 要
基于嗜热链球菌繁殖使牛奶黏度、密度发生改变,从而导致压电晶体频率发生改变,据此提出了应用压电传感器,通过测定频率检测时间(FDT)来间接测定牛奶中抗生素残留量的方法。氨苄青霉素、氯霉素、氧氟沙星和硫酸双氢链霉素检出限(3S/N)分别为0.30,0.36,0.29,0.28 μg·L-1。方法用于测定牛奶中抗生素的含量,并加入氯霉素做回收试验,测得回收率在82.0%~90.4%之间。
Abstract
Based on the changes of dynamic viscosity and density of milk with the breeding of S. thermophilus in milk, which lead to the frequency change of piezoelectric sensor, a new method for the indirect determination of residual amount of antibiotics in milk was proposed using piezoelectric sensor by determination of frequency detection time (FDT). Values of detection limit (3S/N) of ampicillin, chloromycetin, cfloxacin and dihydrostreptomycin sulfate found were 0.30, 0.36, 0.29, 0.28 μg·L-1, respectively. Test for recovery was made on the base of milk samples by addition of chloromycetin standard, giving values of recovery in the range of 82.0%-90.4%.
中图分类号 O652.9
所属栏目 试验与研究
基金项目 2010年江苏省高等学校大学生实践创新训练项目(苏高教(2010)16号)
收稿日期 2012/1/16
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联系人作者袁逸飞(wxy62@cslg.cn)
备注袁逸飞(1990-),男,江苏扬州人,研究方向为化学与生物传感技术。
引用该论文: YUAN Yi-fei,SHEN Mei-jian,ZHANG Long,LI Kang,JIN Li,WANG Xue-ying. Rapid Determination of Residual Amount of Antibiotic in Milk by Piezoelectric Sensor[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(2): 134~137
袁逸飞,沈美健,张龙,李康,靳力,汪学英. 压电传感器快速检测牛奶中抗生素残留量[J]. 理化检验-化学分册, 2013, 49(2): 134~137
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参考文献
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【17】SHEN Da-zhong, ZHU Wen-hong, NIE Li-hua, et al. Behavior of a series piezoelectric sensor in electrolyte solution[J]. Enzy Microb Anal Chim Acta, 1993,276(1):87-97.
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【19】王志强,胡国媛,李志勇,等.微生物抑制法快速检测鲜奶中多种抗生素残留[J].中国食品卫生杂志, 2008,20(2):139-141.
【2】曾伟.奶牛乳房炎综合防治[J].四川畜牧兽医, 2009,36(2):47-49.
【3】张琰图,章竹君,孙永华.高效液相色谱化学发光法检测牛奶中残留四环素类化合物的研究[J].化学学报, 2006,64(24):2461-2466.
【4】张辉.高效液相色谱法测定肉类食品中四环素类抗生素残留[J].理化检验-化学分册, 2004,40(10):609-610.
【5】CHEN Huai-xi, CHEN Hui, YING Jun, et al. Dispersive liquid-liquid microextraction followed by high-performance liquid chromatography as an efficient and sensitive technique for simultaneous determination of chloramphenicol and thiamphenicol in honey[J]. Anal Chim Acta, 2009,632(1):80-85.
【6】钱鸣蓉,章虎,吴俐勤,等.固相萃取样品-高效液相色谱-串联质谱法快速测定蜂蜜中26种抗生素[J].理化检验-化学分册, 2010,46(9):1093-1095.
【7】胡炎明,程雪梅,何太平.高效液相色谱-质谱法测定虾中四环素类抗生素残留量[J].理化检验-化学分册, 2010,46(4):445-447.
【8】KIM N, KIM D K, KIM W Y. Sulfamethazine detection with direct-binding optical waveguide lightmode spectroscopy-based immunosensor[J]. Food Chemistry, 2008,108(2):768-773.
【9】KIM Y K, KIM H. Immuno-strip biosensor system to detect enrofloxacin residues[J]. Journal of Industrial and Engineering Chemistry, 2009,15(2):229-232.
【10】ASKAL H F, REFAAT I H, DARWISH I A , et, al. A selective spectrophotometric method for determination of rosoxacin antibiotic using sodium nitroprusside as a chromogenic reagent[J]. Spectrochimica Acta Part A, 2008,69(4):1287-1291.
【11】柳玲宝,屠一锋.荧光光度法测定抗生素氧氟沙星[J].苏州大学学报:自然科学, 2000,16(2):72-75.
【12】廖玲,何艳玲,刘沛.嗜热脂肪芽孢杆菌检测牛乳中抗生素残留[J].食品工业科技, 2010,31(9):348-351.
【13】郑维君,常炜,马文宏.牛奶中抗生素残留量两种检测方法的比较[J].食品研究与开发, 2010,31(6):158-159.
【14】HE Feng-jiao, ZHOU Juan. A new antimicrobial susceptibility testing method of Escherichia coli against ampicillin by MSPQC[J]. Journal of Microbiological Methods, 2007,68(3):563-567.
【15】周娟,何凤姣.压电传感器在抗生素给药方案中的应用研究[J].湖北大学学报:自然科学版, 2009,31(3):290-293.
【16】姚守拙.非质量效应压电化学传感的应用与发展[J].化学通报, 2001(9):533-536.
【17】SHEN Da-zhong, ZHU Wen-hong, NIE Li-hua, et al. Behavior of a series piezoelectric sensor in electrolyte solution[J]. Enzy Microb Anal Chim Acta, 1993,276(1):87-97.
【18】张波,府伟灵,蒋天伦,等.压电石英晶体传感器液相检测的影响因素[J].第三军医大学学报, 2002,24(1):8-11.
【19】王志强,胡国媛,李志勇,等.微生物抑制法快速检测鲜奶中多种抗生素残留[J].中国食品卫生杂志, 2008,20(2):139-141.
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