新型荧光探针罗丹明肉桂酰胺的合成及其用于荧光分光光度法测定汞
Synthesis of Rhodamine Cinnamide and Its Use as a New Fluorescence Probe in Fluorospectrophotometric Determination of Mercury
摘 要
以罗丹明B和肉桂酸为主要反应物,制备了新型荧光探针罗丹明肉桂酰胺,并采用红外光谱、核磁和质谱对荧光探针的结构进行了表征。在含30%(φ)乙醇的pH 7.0 HEPES缓冲溶液中,荧光探针与Hg2+形成1比1的络合物,通过测定在发射波长584 nm处的荧光强度,与汞的浓度在1.0×10-6mol·L-1以内呈线性关系,方法的检出限(3s)为7.4×10-9mol·L-1。试验结果表明:该荧光探针对Hg2+具有很好的选择性,不受其他金属离子的干扰。
汞
荧光探针
Rhodamine cinnamide
Mercury
Fluorescence probe
Abstract
A new fluorescence probe, rhodamine cinnamide, was synthesized with rhodamine and cinnamic acid; and structure of the probe was characterized by IR, NMR and MS. In a HEPES buffer solution of pH 7.0 containing 30% (φ) ethanol, a complex with molar ratio of 1 to 1 was formed by the reaction of the probe with Hg2+. Linear relationship between values of fluorescence intensity and concentration of Hg2+ was found in the range within 1.0×10-6mol·L-1 measured at the wavelength of 584 nm (λem), with limit of detection of 7.4×10-9mol·L-1. It was found that this fluorescence probe exhibits highly selective toward Hg2+, and the determination of Hg2+ was not interfered by other metal ions.
中图分类号 O657.3
所属栏目 试验与研究
基金项目 国家自然科学基金项目(50973084);高等学校科学技术发展基金(20071214)
收稿日期 2012/2/17
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联系人作者曹东磊(yfany@163.com)
备注曹东磊(1986-),男,河南濮阳人,硕士,主要从事荧光探针及智能材料研究。
引用该论文: CAO Dong-lei,YAN Fan-yong,WANG Meng,WEN Jing-yun,LI Xue-fang,FU Yang,CHEN Li. Synthesis of Rhodamine Cinnamide and Its Use as a New Fluorescence Probe in Fluorospectrophotometric Determination of Mercury[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(2): 138～141
曹东磊,颜范勇,王猛,温景云,李雪芳,付洋,陈莉. 新型荧光探针罗丹明肉桂酰胺的合成及其用于荧光分光光度法测定汞[J]. 理化检验－化学分册, 2013, 49(2): 138～141
被引情况:
【1】王昊,熊斌,周红审,王永祥,程华, "荧光分析技术在卷烟烟气成分检测中的研究进展",理化检验－化学分册 50, 1609-1612(2014)
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参考文献
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【2】HARRIS H H, PICKERING I J, GEORGE G N. The chemicalform of mercury in fish［J］. Science, 2003,301:1203-1203.
【3】AMINI M K, KHEZRI B, FIROOZ A R. Development of a highly sensitive and selective optical chemical sensor for batch and flow-through determination of mercury ion［J］. Sens Actuators B: Chem, 2008,131:470-478.
【4】GUO T, BAASNER J, GRADL M, et al. Determination of mercury in saliva with a flow-injection system［J］. Anal Chim Acta, 1996,320:171-176.
【5】MUTHUKUMAR C, KESARKAR S D, SRIVASTAVA D N. Conductometric mercury(Ⅱ) sensor based on polyaniline-cryptand-222 hybrid［J］. J Electroanal Chem, 2007,602:172-180.
【6】刘沛,刘盛余,叶芝祥,等.原子荧光光谱法测定环境水样中痕量汞［J］.理化检验-化学分册, 2009,45(11):1326-1328.
【7】CALLAN J F, DE SILVA A P, MAGRI D C. Luminescent sensors and switches in the early 21st century［J］. Tetrahedron, 2005,61:8551-8588.
【8】HAUPT K, MOSBACH K. Molecularly imprinted polymers and their use in biomimetic sensors［J］. Chem Rev, 2000,100(7):2495-2504.
【9】王晓春,刘晓端,杨永亮,等.罗丹明类荧光探针在重金属和过渡金属离子检测中的应用［J］.光谱学与光谱分析, 2010,30(10):2693-2699.
【10】NOLAN E M, LIPPARD S J. Tools and tactics for the optical detection of Mercuric ion［J］. Chem Rev, 2008,108:3443-3480.
【11】DOMAILLE D W, QUE E L, CHANG C J. Synthetic fluorescent sensors for studying the cell biology of metals［J］. Nat Chem Biol, 2008,4:168-175.
【12】王文革,赵书林,李舒婷.1-偶氮苯-3-(6-甲氧基-2-苯并噻唑)-三氮烯的合成及用于汞的光度测定［J］.理化检验-化学分册, 2006,42(2):100-102.
【13】WETZEL A, EHRHARDT V, HEINRICH M R. Synthesis of amino-and hydroxybiphenyls by radical chain reaction of arenediazonium salts［J］. Angew Chem Int Ed, 2008,47:9130-9133.
【14】ZHANG Xuan, SHIRAISHI Y, HIRAI T. Cu(Ⅱ)-selective green fluorescence of a rhodamine-diacetic acid conjugate［J］. Org Lett, 2007,9:5039-5042.
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